@misc{10481/106787,
year = {2025},
month = {9},
url = {https://hdl.handle.net/10481/106787},
abstract = {Avocado is a unique fruit in which of seven-carbon (C7) sugars (notably D-mannoheptulose
and perseitol) dominate the carbohydrate profile at harvest. Despite growing interest in
sugar-mediated ripening processes, limited comparative data exist across cultivars. This
work characterises the dynamic changes in non-structural carbohydrates in the mesotecarp of three commercially relevant avocado varieties—Bacon, Fuerte, and Hass—across
four defined ripening stages, from unripe to overripe, with five biological replicates per
stage. Using a validated hydrophilic interaction liquid chromatography–mass spectrometry
(HILIC–MS) method, we quantified five key sugars and assessed their evolution through
ripening. Concentrations varied among the studied samples within the following ranges:
D-mannoheptulose, 0.4–49 mg/g dry weight (DW); perseitol, 0.5–23 mg/g DW; glucose,
0.8–5.3 mg/g DW; fructose, 0.6–4.5 mg/g DW; and sucrose, 0.5–3.4 mg/g DW. C7 sugar
levels consistently declined, while C6 sugars increased—primarily between the intermediate and ready-to-eat stages—with distinct cultivar-specific patterns. Bacon maintained
elevated C7 concentrations for a longer period; Fuerte exhibited a rapid transition from C7
to C6 sugars; and Hass displayed a more gradual and balanced shift. Multivariate analysis
(partial least squares discriminant analysis, PLS-DA) effectively discriminated between
cultivars at each ripening stage, confirming cultivar-specific metabolic signatures. These
findings offer new insights into avocado carbohydrate metabolism, emphasising varietydependent pathways that could inform breeding strategies, optimise postharvest ripening
protocols, and support the nutritional characterisation of different avocado cultivars.},
organization = {MICIU/AEI/10.13039/501100011033 y FEDER 
(PID2021-128508OB-I00, PCI2024-153520)},
organization = {ANID — Fondecyt
(1220223)},
organization = {ANID - Millennium Science Initiative Programme (ICN2021_044)},
organization = {Spanish Government
(PRE2022-101406)},
organization = {MICIU/AEI/ - ESF+ (JDC2023-052792-I)},
publisher = {MDPI},
keywords = {avocado ripening},
keywords = {non-structural carbohydrates},
keywords = {hydrophilic interaction chromatography−mass spectrometry},
title = {Cultivar-Specific Differences in C6 and C7 Sugar Metabolism During Avocado Ripening: Comparative Insights from Bacon, Fuerte, and Hass},
doi = {10.3390/plants14182856},
author = {Beiro-Valenzuela, María Gemma and P. Monasterio, Romina and Serrano-García, Irene and Hurtado-Fernández, Elena and Sánchez-Arévalo, Carmen María and Fernández-Sánchez, Jorge Fernando and Pedreschi, Romina and Olmo-García, Lucía and Carrasco-Pancorbo, Alegría},
}