@misc{10481/106619,
year = {2025},
month = {9},
url = {https://hdl.handle.net/10481/106619},
abstract = {Intracellular copper-catalyzed azide-alkyne cycloaddition (CuAAC) offers
immense potential for bioorthogonal chemistry, but its application is severely
hindered by copper toxicity and the challenge of controlling catalysis within
the complex cellular environment. Heterogeneous copper catalysts can reduce
toxicity by minimizing free copper exposure and enabling localized activity, yet
optimizing their performance in situ within living cells remains a significant
hurdle. Here, the development of a novel dual-functional nanocatalyst,
Cu@BTTAA-Cy5-NPs, that combines robust heterogeneous CuAAC catalytic
activity with intrinsic fluorescence tracking is reported. The successful
synthesis and characterization of these monodispersed nanoparticles is
demonstrated, confirming efficient copper loading stabilized by BTTAA and
the nanoparticle matrix, and critically, the retention of Cy5 fluorescence for
tracking. This unique dual functionality allows for real-time monitoring of
nanoparticle localization and correlation with catalytic product formation via
distinct fluorescence channels, enabling, for the first time to our knowledge,
comprehensive in situ screening and optimization of CuAAC reaction
conditions directly within living cells using fluorescence feedback. The
nanoparticles exhibit excellent biocompatibility and cellular uptake, showing
no significant toxicity, apoptosis, or oxidative stress at active concentrations.},
organization = {European Union Next Generation EU/PRTR. (Grant Numbers: PDC2022.133913.I00, PID2022-141065OB-I00, RED2022-134560-T)},
organization = {Consejería de Salud y Consumo. (Grant Numbers: PIP-0232-2021, PIP-0245-2024)},
organization = {GENYO Centre for Genomics and Oncological Research},
organization = {Pfizer},
organization = {Andalusian Regional Government. (Grant Number: POSTDOC_21_00118)},
publisher = {MDPI},
title = {Tracker Nanocatalyst for Screening of Intracellular Copper-Catalyzed Azide-Alkyne Cycloadditions},
doi = {10.1002/smll.202506185},
author = {Rodríguez-Segura, Mónica and López-Delgado, Francisco Javier and Cano-Cortés, María Victoria and Delgado-González, Antonio and Díaz-Mochón, Juan José and Sánchez-Martín, Rosario María},
}