@misc{10481/106594,
year = {2025},
month = {8},
url = {https://hdl.handle.net/10481/106594},
abstract = {Several distantly related, deep-branching lineages within the eukaryotic tree of life share what is known as the “typical excavate”
morphology. This cell architecture includes a suspension-feeding ventral groove, supported by complex and specific structures
of the cytoskeleton, and a vane-bearing posterior flagellum. Within Discoba, such characteristics were found only in the deepest
branch, Jakobida, also famous for having the most gene-rich and bacterial-like mitogenomes. Some members of the discoban
lineage Heterolobosea also possess a ventral groove. However, they lost many other typical excavate characteristics, including
the flagellar vane and some flagellar root elements. We have established a clonal culture of a free-living freshwater flagellate
from Malaysia, strain NY0229, described here under the provisional name Multisulcus malaysiensis nom. prov. Electron microscopy observations show a conspicuous ventral groove reinforced with microtubules of the flagellar apparatus and a vane on the
posterior flagellum. Strain NY0229 has typical excavate biflagellated forms but also exhibits very atypical multiflagellated and
multigrooved forms. The kinetid consists of two orthogonally oriented kinetosomes; both flagella of biflagellate cells display
proximal and distal projections. Molecular phylogenetic analyses support a deep-branching position of the new strain within
Heterolobosea, as the sister lineage to all previously known Eutetramitia.},
organization = {European Research Council (Grant Numbers: 787904, 101141745)},
organization = {Ramón y Cajal Programme (Grant Number: RYC2022-035282-I)},
publisher = {Wiley Periodicals LLC},
keywords = {Electron microscopy},
keywords = {Heterolobosea},
keywords = {Karyomastigont},
title = {A Deeply Branching Lineage in Heterolobosea (Discoba) With Multiflagellated and Multigrooved Life Stages},
doi = {10.1111/jeu.70037},
author = {Prokina, Kristina I. and Yubuki, Naoji and Galindo, Luis Javier and Torruella, Guifré and Inagaki, Yuji and Iwamoto, Koji and López-García, Purificación and Moreira, David},
}