@misc{10481/106048,
year = {2024},
url = {https://hdl.handle.net/10481/106048},
abstract = {Objectives
Tideglusib has shown great performance in terms of dentin regenerative properties. This study aims to evaluate bonding ability, of demineralized dentin infiltrated with polymeric nanoparticles (NPs) doped with tideglusib (TG) (TG-NPs).
Methods
Dentin conditioned surfaces were infiltrated with NPs and TG-NPs. Bonded interfaces were created and stored for 24 h and then submitted to mechanical, chemical and thermal challenging. The resin-dentin interface was evaluated through a doubled dye fluorescent technique and a calcium chelator fluorophore under a confocal laser scanning microscopy, and by field emission scanning electron microscopy.
Results
Dentin surfaces treated with TG-NPs and load cycled produced higher bond strength than the rest of the groups. Immersion of dentin specimens treated with undoped-NPs in collagenase solution attained the lowest microtensile bond strength (MTBS) values. Both porosity and nanoleakage decreased when dentin was infiltrated with TG-NPs, that revealed strong signals of xylenol orange stain at both hybrid layer and dentinal tubules. The presence of NPs, in general, inducted the presence of mineralized interfaces after mechanical loading and thermocycling.
Conclusions
Nanoparticles doped with tideglusib promoted the highest dentin bonding efficacy among groups, as they facilitated the maximum bond strength values with creation of mineral deposits at the hybrid layer and dentinal walls. Tideglusib enabled scarce porosity, nanoleakage and advanced sealing among dentin groups.
Significance
Doping hydrophilic polymeric NPs with tideglusib, infiltrated in etched dentin represents a reproducible technique to create reparative dentin at the resin-dentin interface, by inducing therapeutic bioactivity.},
organization = {MCIN/AEI 10.13039/501100011033  PID2020–114694RB-I00},
organization = {University of Granada},
publisher = {Elsevier},
keywords = {Dentin},
keywords = {Tideglusib.},
keywords = {Mechanical},
keywords = {Collagenase},
keywords = {Nanoparticles},
keywords = {Remineralization},
keywords = {Confocal microscopy},
keywords = {Microtensile bond strength},
keywords = {Scanning electron microscopy},
title = {Dentin remineralization using a stimuli-responsive engineered small molecule GSK3 antagonists-functionalized adhesive},
doi = {10.1016/j.dental.2023.12.010},
author = {Toledano Pérez, Manuel and Sánchez Aguilera, Fátima and Fernández-Romero, Enrique and Lagos, Alejandro J. and Bonilla, Marcos and Lynch, Christopher D. and Osorio Ruiz, Raquel},
}