@misc{10481/105888,
year = {2025},
month = {7},
url = {https://hdl.handle.net/10481/105888},
abstract = {A significant fraction of the genomes of most multicellular eukaryotes includes extensive arrays of tandemly repeated sequences,
collectively referred to as satellite DNAs (satDNAs). However, the mechanisms responsible for generating and maintaining
varying satDNA abundances across lineages and temporal scales are still unclear. This work focused on arowana fishes (Teleostei,
Osteoglossiformes) as a model; their widespread intercontinental distribution and basal phylogenetic position within Teleostei
make them a compelling model for evolutionary research, especially in the realm of satDNA molecular evolution. Through the
integration of genomic and chromosomal data, we analyzed and compared the catalogs of satDNA families (i.e., satellitomes)
of four out of the six extant arowana species, elucidating ancestral evolutionary trends and establishing their temporal history.
Arowanas displayed a small number of satDNA families, ranging from 16 to 25 in Osteoglossum bicirrhosum and Scleropages
formosus, respectively. Alongside the identification of some shared satDNAs, many considered species-specific, nonetheless
possess a limited number of copies in other species. The minimal variation observed both within and across species highlights the
long-term conservation of satDNAs during evolution, since specific ones (referred to as long-term conserved satDNAs) may have
endured throughout a lengthy evolutionary period. Moreover, fluorescence in situ hybridization (FISH) investigations conducted
with the most abundant satDNAs demonstrated unique hybridization patterns for homologous orthologous ones, signifying their
dynamic genomic positioning. Besides, the similarities of satDNAs among species align with their phylogenetic relationships,
showing the high dynamism of arowanas’ satDNAs, with several evolutionary events driving their sequence diversity},
organization = {São Paulo Research Foundation (FAPESP) -  [grant 2021/11169-2 (FHSS), 2022/00427-3 (GAT), and 2023/00955-2 (MBC)]},
organization = {Brazilian National Council for Scientific and Technological Development (CNPq) - grant number 302928/2021-9 (M.B.C.)},
organization = {Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil (CAPES)},
organization = {DEAL (Deutsche Forschungsgemeinschaft = DFG, Germany)},
publisher = {Wiley},
keywords = {FISH},
keywords = {Fishes},
keywords = {Library hypothesis},
keywords = {Repetitive DNAs},
keywords = {Satellitome},
title = {Comparative Satellitomics in Arowanas (Telostei, Osteoglossiformes) Sheds Light on the Evolution of Ancient Satellite DNAs},
doi = {10.1111/1749-4877.13008},
author = {Santos de Souza, Fernando Henrique and Akira Toma, Gustavo and Dziechciarz Vidal, Jhon Alex and Garrido Ramos, Manuel Ángel and Mota Souza, Guilherme and Zeni Dos Santos, Rodrigo and Porto-Foresti, Fábio and Liehr, Thomas and Utsunomia, Ricardo and de Bello Cioffi, Marcelo},
}