@misc{10481/105721,
year = {2025},
month = {7},
url = {https://hdl.handle.net/10481/105721},
abstract = {Black Chiloe’s giant garlic is a functional food produced by a mild Maillard reaction that contains relevant bioactive molecules like organosulfur compounds (OSCs) and (poly)phenols
(PPs). Compared with raw garlic, black garlic has a higher content of PPs and S-allyl
cysteine (SAC), a key OSC due to its bioactivities. The objective of the present work was to
optimize by chemometric tools a green microwave-assisted extraction (MAE) of SAC and
PPs present in black Chiloe’s giant garlic to detect and identify novel bioactive molecules
with antioxidant and/or inhibitory activities over cyclooxygenase, α-glucosidase, and
acetylcholinesterase enzymes. The MAE factors were optimized using a central composite
design, establishing optimal PP and SAC yields at 67 ◦C, 0% ethanol, 12 min and 30 ◦C,
40% ethanol, 3 min, respectively. PP and SAC values were 9.19 ± 0.18 mg GAE/g DW and
2.55 ± 0.10 mg SAC/g DW. Applying effect-directed analysis using high-performance thin
layer chromatography-bioassay and mass spectrometry, the bioactive molecules present
in the MAE extract with antioxidant and inhibitory activities over cyclooxygenase, αglucosidase, and acetylcholinesterase enzymes were identified as N-fructosyl-glutamyl-S-
(1-propenyl)cysteine, N-fructosyl-glutamylphenylalanine, and Harmane.},
organization = {FONDECYT (projects No. 1201658 and 1211803)},
organization = {International Cooperation (project No. FOVI210081)},
organization = {MCIN/AEI/10.13039/501100011033 - FEDER, UE (FONDEQUIP EQM210203,  EQY220018,  PID2021-125188OB-C31)},
publisher = {MDPI},
keywords = {MAE},
keywords = {(poly)phenols},
keywords = {planar chromatography},
keywords = {S-allyl-cysteine},
keywords = {cyclooxygenase},
keywords = {α-glucosidase},
keywords = {acetylcholinesterase},
title = {Identification of Novel Bioactive Molecules in Black Chiloe’s Giant Garlic (Allium ampeloprasum L.) by Green MicrowaveAssisted Extraction and Effect-Directed Analysis Using High-Performance Thin Layer Chromatography-Bioassay and Mass Spectrometry},
doi = {10.3390/antiox14080913},
author = {Fernández-Martínez, Joaquín and Arráez-Román, David and Peterssen, Darlene and Zapata, Gerald and Henríquez-Aedo, Karem and Aranda, Mario},
}