@misc{10481/105660,
year = {2012},
month = {9},
url = {https://hdl.handle.net/10481/105660},
abstract = {Introduction: Olea europaea L. leaves may be considered a cheap, easily available natural source of phenolic compounds. In a previous study we evaluated the possibility of obtaining bioactive phenolic compounds from olive leaves by pressurised liquid extraction (PLE) for their use as natural anti-oxidants. The alimentary use of these kinds of extract makes comprehensive knowledge of their composition essential.
Objective: To undertake a comprehensive characterisation of two olive-leaf extracts obtained by PLE using high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS).
Method: Olive leaves were extracted by PLE using ethanol and water as extraction solvents at 150°C and 200°C respectively. Separation was carried out in a HPLC system equipped with a C₁₈-column working in a gradient elution programme coupled to ESI-QTOF-MS operating in negative ion mode.
Results: This analytical platform was able to detect 48 compounds and tentatively identify 31 different phenolic compounds in these extracts, including secoiridoids, simple phenols, flavonoids, cinnamic-acid derivatives and benzoic acids. Lucidumoside C was also identified for the first time in olive leaves.
Conclusion: The coupling of HPLC-ESI-QTOF-MS led to the in-depth characterisation of the olive-leaf extracts on the basis of mass accuracy, true isotopic pattern and tandem mass spectrometry (MS/MS) spectra. We may conclude therefore that this analytical tool is very valuable in the study of phenolic compounds in plant matrices.},
organization = {This work was supported by the projects AGL2008-05108-C03-03/01 and AGL2011-29857-C03-02 of the Spanish Ministry of Science and Innovation; P09-CTS-4564, P10-FQM-6563 and P11-CTS-7625 of the Andalusian Regional Government Council of Innovation and Science, and GREIB.PT.2011.18. The authors are grateful to the Spanish Ministry of Science and Innovation for FPU grant AP2007-03246 and a ‘Ramón y Cajal’ research contract.},
publisher = {Wiley},
keywords = {Electrospray ionisation},
keywords = {High-performance liquid chromatography},
keywords = {Quadrupole time-of-flight},
keywords = {Olive leaves},
keywords = {Phenolic compounds},
title = {HPLC–ESI–QTOF–MS as a Powerful Analytical Tool for Characterising Phenolic Compounds in Olive-leaf Extracts},
doi = {10.1002/pca.2401},
author = {Quirantes-Piné, Rosa and Lozano-Sánchez, Jesús and Herrero, Miguel and Ibáñez, Elena and Segura Carretero, Antonio and Fernández-Gutiérrez, Alberto},
}