@misc{10481/105093,
year = {2025},
month = {6},
url = {https://hdl.handle.net/10481/105093},
abstract = {Spent coffee grounds (SCGs) are a form of biowaste that can be reused in agronomic biofortification with
Zn and Fe to enhance environmental sustainability. Various chemical treatments of SCGs have been
explored to reduce their known phytotoxicity. The aim of this study was to evaluate how different
chemically modified SCGs used as soil amendments for iron (Fe) and zinc (Zn) biofortification of Dutch
cucumbers under greenhouse conditions influence the accumulation of arsenic (As), cadmium (Cd),
mercury (Hg), and lead (Pb). Cucumbers grown with SCGs activated with sodium hydroxide (NaOH)
(ASCG) and all SCG-based biochelates showed reduced Cd concentrations compared to the control group
(p < 0.05). Activated hydrochar derived from SCGs and functionalized with Fe (biochelate: AH160-Fe;
0.027 ± 0.019 μgg kkgg−1, fresh weight, f.w), or ASCG functionalized with Zn (biochelate: ASCG-Zn;
0.033 ± 0.025 μgg kkgg−1), reduced Hg levels relative to the control (0.050 ± 0.035 μgg kkgg−1;
p < 0.05). Activated hydrochar produced from SCGs heated at 160 °C (AH160) decreased As
concentrations (7.69 ± 1.41 μgg kkgg−1) compared to commercial Zn and Fe chelates (control-Zn:
9.57 ± 1.70 μgg kkgg−1 and control-Fe: 8.28 ± 1.38 μgg kkgg−1, respectively; p < 0.05). AH160
functionalized with Zn (biochelate AH160-Zn) and Fe (biochelate AH160-Fe) reduced As and Hg
concentrations (8.51 ± 2.34 and 0.027 ± 0.019 μgg kkgg−1, respectively) compared to control-Zn and
control-Fe (9.57 ± 1.70 and 0.049 ± 0.041 μgg kkgg−1, respectively; p < 0.05). Cucumbers harvested at
later stages exhibited higher As and Cd levels, in contrast to Hg and Pb, which decreased over time. In
conclusion, the four biochelates (ASCG-Zn, AH160-Zn, ASCG-Fe, and AH160-Fe) reduced Cd
bioavailability in cucumbers (0.120, 0.126, 0.122, and 0.136 μgg kkgg−1, respectively), with ASCG-Zn
and ASCG-Fe showing the most significant effect compared to the control (0.156 μgg kkgg−1). The tested
biochelates present a promising alternative to commercial Zn and Fe chelates, enabling biofortification while
simultaneously lowering Cd, Hg, and As levels. In subsequent harvests, As and Cd levels tend to increase,
whereas Hg and Pb levels decrease. None of the estimated daily intakes of the analyzed toxic metals
(0.0439, 0.0007, 0.0002, and 0.0027 μg day−1 for As, Cd, Hg, and Pb, respectively) pose a risk to human
health.},
organization = {Gobierno de Andalucía (España) P20_00585},
publisher = {Elsevier},
keywords = {As–Cd–Hg–Pb},
keywords = {Bioaccumulation},
keywords = {Estimated daily intake},
title = {Levels of toxic metals in Dutch cucumbers biofortified (Fe–Zn) with chemically-modified spent coffee grounds},
doi = {10.1016/j.chemosphere.2025.144550},
author = {Navarro-Moreno, Miguel and Cervera Mata, Ana Gloria and Fernández Arteaga, Alejandro and Pastoriza de la Cueva, Silvia and Lara-Ramos, Leslie and Delgado Calvo-Flores, Gabriel and Navarro Alarcón, Miguel and Rufián Henares, José Ángel},
}