@misc{10481/104113,
year = {2025},
month = {4},
url = {https://hdl.handle.net/10481/104113},
abstract = {Melatonin is an indolamine derived from tryptophan, which is highly conserved
throughout evolution, including in zebrafish, where it controls important cellular
processes, such as circadian rhythms, oxidative stress, inflammation, and mitochondrial
homeostasis. These functions of melatonin and its synthesis route are quite similar to
those in humans. One of the most important enzymes in melatonin synthesis is acetylserotonin
O-methyltransferase (ASMT), the rate-limiting enzyme, which catalyzes its final
step. Due to genome duplication, zebrafish has two genes for this enzyme, asmt1 and
asmt2. These genes show differential expression; asmt1 is primarily expressed in the retina
and the pineal gland, and asmt2 is expressed in peripheral tissues, indicating different
functions. Therefore, the aim of this work was to develop a mutant model for each asmt
gene and to analyze their phenotypic effects in zebrafish. The results showed that the loss
of 80% of the asmt2 gene affected melatonin concentration and consequently disrupted the
sleep/wake rhythm in larvae, decreasing by 50% the distance traveled. In contrast, the loss
of asmt1 had a greater influence on the physical condition of adults, as locomotor activity
decreased by 50%, and 75% showed malformations. These data reveal distinct functional
roles of melatonin depending on their site of production that may affect the development
of zebrafish.},
organization = {Grants no. A-CTS.205.UGR18 from the Projects I+D+i
Programa Operativo FEDER Andalucía 2014–2020, P18-RT-698 from PAIDI 2020, Junta de Andalucía,
CB16-10-00238 (ISCIII, co-funded by the European Regional Development Fund/European Social
Fund “Investing in your future”)},
organization = {Consejería de Innovación, Ciencia y Empresa, Junta de
Andalucía (CTS-101)},
organization = {Grant no. P18-RT-698},
organization = {FPU fellowships from the Ministerio de Educación},
organization = {Grant no. PI19-01372 (FIS, ISCIII, Spain)},
publisher = {MDPI},
keywords = {Zebrafish},
keywords = {Asmt1},
keywords = {Asmt2},
title = {Lack of asmt1 or asmt2 Yields Different Phenotypes and Malformations in Larvae to Adult Zebrafish},
doi = {10.3390/ijms26083912},
author = {Aranda Martínez, Paula and Fernández Martínez, José and Díaz Casado, María Elena and Ramírez Casas, Yolanda and Martín-Estebané, María and López Rodríguez, Alba and Escames Rosa, Germaine and Acuña Castroviejo, Darío},
}