@misc{10481/103873,
year = {2025},
month = {3},
url = {https://hdl.handle.net/10481/103873},
abstract = {Background

Soluble Alpha-Klotho (S-αklotho) protein and 25-Hydroxyvitamin D (25-OH-D) have emerged as potential modulators for activating and recruiting Brown Adipose Tissue (BAT). The present study aimed to investigate whether circulating S-αklotho and 25-OH-D levels are related to BAT volume, 18Fluorine-Fluorodeoxyglucose (18F-FDG) uptake, and BAT radiodensity in young healthy adults.
Methods

A total of 128 participants (68% women) aged 18–25 years old participated in this cross-sectional study. Serum levels of S-αklotho were determined by a solid-phase sandwich enzyme-linked immunosorbent assay kit and 25-OH-D serum levels were analyzed using a competitive chemiluminescence immunoassay, both in blood samples collected after an overnight fast. All participants underwent a personalized cold exposure to determine their BAT volume, 18F-FDG uptake, and radiodensity, using a static positron emission tomography combined with computed tomography scan.
Results

After adjusting for multiple covariates, serum levels of S-αklotho (all R2 ≤ 0.228 and P ≥ 0.364), 25-OH-D as continuous (all R2 ≤ 0.242 and P ≥ 0.088) or by vitamin D status (all R2 ≤ 0.767 and P ≥ 0.061) were not associated with either BAT volume and 18F-FDG uptake, or BAT radiodensity.
Conclusion

Serum S-αklotho and 25-OH-D levels within the physiological range are not related to BAT-related variables in young healthy adults. Further studies are needed to fully understand the underlying mechanisms involved in BAT metabolism in humans. (ACTIBATE; ClinicalTrials.gov identifier: not applicable).},
organization = {Spanish Ministry of Economy and Competitiveness Instituto de Salud Carlos III (PI13/01393), PTA-12264, (DEP2016-79512-R)},
organization = {European Regional Development Funds (ERDF)},
organization = {Spanish Ministry of Education (FPU13/04365 and FPU14/04172)},
organization = {Fundación Iberoamericana de Nutrición (FINUT)},
organization = {Redes Temáticas de Investigación Cooperativa RETIC (Red SAMID RD16/0022)},
organization = {AstraZeneca HealthCare Foundation},
organization = {University of Granada},
organization = {Junta de Andalucía},
organization = {Fundación Alfonso Martin Escudero},
organization = {CIBEROBN, Centro de Investigación Biomédica en Red (CB22/03/00058)},
organization = {Instituto de Salud Carlos III},
organization = {Ministerio de Ciencia e Innovación},
organization = {Unión Europea – European Regional Development Fund},
organization = {Universidad de Granada/CBUA},
publisher = {Springer Nature},
keywords = {Klotho protein},
keywords = {25-Hydroxyvitamin D},
keywords = {Ageing},
keywords = {Brown adipose tissue},
title = {Soluble alpha‑klotho and 25‑hydroxivitamin D are not associated with brown adipose tissue metabolism in young healthy adults},
doi = {10.1007/s13105-025-01072-z},
author = {Amaro Gahete, Francisco José and Vázquez Lorente, Héctor and Sánchez Delgado, Guillermo and Ruiz Ruiz, Jonatan},
}