@misc{10481/103006,
year = {2025},
month = {2},
url = {https://hdl.handle.net/10481/103006},
abstract = {To overcome the limitations of conventional skin tissue engineering (TE), 3D biofabrication approaches are being
developed. However, tissue mimicry should be further improved in skin models. Here, we developed and
characterized biomimetic hydrogels to obtain a biofabricated three-layered (BT) skin substitute based on the
main components found in the epidermal, dermal, and hypodermal skin layers. Hydrogels for dermal and hypodermal
skin layers were based on a mix of agarose and type I collagen, supplemented with skin-related
extracellular matrix (ECM) components (dermatan sulfate, hyaluronic acid, and elastin) and loaded with
human dermal fibroblasts (hDFs) or human mesenchymal stem/stromal cells (hMSCs), respectively. The
epidermal hydrogel was formulated using type I collagen supplemented with keratin and sphingolipids, and
seeded with human epidermal keratinocytes (hEKs). Physicochemical results revealed adequate viscosity, gelling
times, and pH for each hydrogel solution. The BT Skin also showed good swelling and degradation kinetics, and
mechanical properties in a similar range of human skin. The hydrogels and BT Skin demonstrated stable cell
viability and metabolic activity, as well as intercellular communication through the release of growth factors.
Moreover, the BT Skin demonstrated controlled inflammation in vivo, and produced results comparable to
autografting in a mouse skin wound model. This bioactive and biomimetic three-layered BT Skin has a
composition that attempts to mimic the natural ECM of the skin, formulated with the characteristic cells and
biomolecules present in each skin layer, and offers promising properties for its clinical application in the
treatment of patients with skin injuries.},
organization = {Ministry of Economy and Competitiveness, Instituto de Salud Carlos III (FEDER funds)
	DTS19/00143	
	PID2019-104883 GB-I00	
	TED2021-129384B-C22},
organization = {Junta de Andalucia
	P18-FR-2470	
	A-CTS-180-UGR20	
	PYC20.RE.015.UGR},
organization = {European Union (EU)
	101092269},
organization = {Modeling Nature (MNat)
	QUAL21-11	
	DTS21/00098	
	B-CTS-230-UGR18},
publisher = {Elsevier},
title = {A bioactive three-layered skin substitute based on ECM components effectively promotes skin wound healing and regeneration},
doi = {10.1016/j.mtbio.2025.101592},
author = {Chocarro-Wrona, Carlos and Pleguezuelos Beltrán, Paula and López de Andrés, Julia and Antich, Cristina and Vicente Álvarez-Manzaneda, Juan De and Jiménez González, Gema and Arias Santiago, Salvador Antonio and Gálvez-Martín, Patricia and López-Ruiz, Elena and Marchal Corrales, Juan Antonio},
}