@misc{10481/102962,
year = {2024},
month = {2},
url = {https://hdl.handle.net/10481/102962},
abstract = {Background: Crocodilians are one of the oldest extant vertebrate lineages, exhibiting a combination of evolutionary success and morphological resilience that has persisted throughout the history of life on Earth. This ability to endure over such a long geological time span is of great evolutionary importance. Here, we have utilized the combination of genomic and chromosomal data to identify and compare the full catalogs of satellite DNA families (satDNAs, i.e., the satellitomes) of 5 out of the 8 extant Alligatoridae species. As crocodilian genomes reveal ancestral patterns of evolution, by employing this multispecies data collection, we can investigate and assess how satDNA families evolve over time.
Results: Alligators and caimans displayed a small number of satDNA families, ranging from 3 to 13 satDNAs in A. sinensis and C. latirostris, respectively. Together with little variation both within and between species it highlighted long-term conservation of satDNA elements throughout evolution. Furthermore, we traced the origin of the ancestral forms of all satDNAs belonging to the common ancestor of Caimaninae and Alligatorinae. Fluorescence in situ experiments showed distinct hybridization patterns for identical orthologous satDNAs, indicating their dynamic genomic placement.
Conclusions: Alligators and caimans possess one of the smallest satDNA libraries ever reported, comprising only four sets of satDNAs that are shared by all species. Besides, our findings indicated limited intraspecific variation in satellite DNA, suggesting that the majority of new satellite sequences likely evolved from pre-existing ones.},
organization = {Open Access funding enabled and organized by Projekt DEAL. V.C.S.O was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (401036/2022–7). M.A. was supported by the Charles University Research Centre program No. UNCE/24/SCI/006 and by Czech Science Foundation Project No. 20-27236 J. M.d.B.C. was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (302928/2021–9) and by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) (2023/00955–2). M.d.B.C. and T.L. were supported by Alexander von Humboldt Foundation (Research Group Linkage Programme). We acknowledge support by the German Research Foundation Projekt-Nr. 512648189 (T.L.) and the Open Access Publication Fund of the Thueringer Universitaets- und Landesbibliothek Jena. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil (CAPES), Finance Code 001.},
publisher = {BioMed Central Ltd},
keywords = {Repetitive DNA},
keywords = {Reptiles},
keywords = {Library hypothesis},
title = {Evolution of ancient satellite DNAs in extant alligators and caimans (Crocodylia, Reptilia)},
doi = {https://doi.org/10.1186/s12915-024-01847-8},
author = {Sales-Oliveira, Vanessa C. and Dos santos, Rodrigo Zeni and Gomes Goes, Caio Augusto and Calegari, Rodrigo Milan and Garrido Ramos, Manuel Ángel and Altmanová, Marie and Ezaz, Tariq and Liehr, Thomas and Porto-Foresti, Fábio and Utsunomia, Ricardo and Cioffi, Marcelo B.},
}