@misc{10481/101879,
year = {2011},
url = {https://hdl.handle.net/10481/101879},
abstract = {Microspheres based on the interaction between 5-amino salicylic acid (5-ASA) and chitosan were
designed as a new colonic delivery system. Microspheres were prepared by a spray drying technique
and characterised by physical properties, morphology and drug dissolution characteristics. Effects of
the microspheres over inflamed Caco-2 cells were also evaluated, by determining cell viability and
expression of mRNA levels of biomarkers involved in the pathogenesis of Inflammatory Bowel Diseases
(IBD) (IL-1beta and IL-8). Results showed that chitosan improved dissolution properties of 5-ASA.
The amount of free drug released over 120 min did not exceed 9mg/cm2, while microspheres showed a
high dissolution rate reaching approximately 50mg/cm2 of drug released. Contact of inflamed cells with
free 5-ASA reduced mRNA levels of IL-1beta and IL-8. Microspheres did not show cytotoxic activity and
maintained ILs levels observed in non-inflamed cells, mimicking the anti-inflammatory effect of 5-ASA
alone.},
keywords = {5-ASA},
keywords = {Chitosan},
keywords = {Cytokines},
keywords = {Inflammation},
keywords = {Drug release},
keywords = {Spray drying},
title = {Assessement of anti-inflammatory properties of microspheres prepared with chitosan and 5-amino salicylic acid over inflamed caco-2 cells},
doi = {10.1016/j.carbpol.2011.03.027},
author = {Aguzzi, Carola and Ortega, Ángeles and Bonferoni, Maria Cristina and Sandri, Giuseppina and Cerezo González, María Pilar and Salcedo, Inmaculada and Sánchez Espejo, Rita María and Viseras Iborra, César Antonio and Caramella, Carla},
}