@misc{10481/101732,
year = {2004},
url = {https://hdl.handle.net/10481/101732},
abstract = {Infection of human T lymphocytes with the Herpesvirus saimiri (HVS) yields immortalized T-cell lines (HVS-T) which retain all the phenotypical and functional characteristics of their parental cells. This represents a new experimental model for studying genetic disorders of T lymphocytes. In spite of the efforts of many laboratories, no satisfactory way has been found so far to modify HVS-T cells genetically. We have analyzed the capacity of oncoretroviral (MLV)- and lentiviral (HIV-1)-based vectors pseudotyped with vesicular stomatitis virus glycoprotein (VSVg) to transduce HVS-T cells. HIV-1-derived vectors efficiently transduced HVS-T cell lines, reaching up to 85% of cells expressing the transgene in a single round of infection. MLV-based vectors, on the other hand, were unable to transduce more than 1% of any of the HVS-T cell lines analyzed. Lentiviral-driven gene expression was maintained constant and stable in HVS-T cells for a minimum of 48 days. We also observed that although the lentiviral transduction efficiency achieved on HVS-T cells is lower than that obtained with tumor or primary endothelial cells, it is nevertheless similar to that found with activated primary T cells.},
organization = {European Union  QLT-1999-01090},
organization = {Spanish Ministry of Health  FIS01/3143},
organization = {Spanish Ministry of Education and Culture},
publisher = {Springer Nature},
keywords = {gene therapy},
keywords = {vectors},
keywords = {Lentiviral vectors},
keywords = {T cells},
keywords = {Heresvirus saimiri},
keywords = {model},
title = {Efficient lentiviral transduction of Herpesvirus saimiri immortalized T cells as a model for gene therapy in primary immunodeficiencies},
doi = {10.1038/sj.gt.3302259},
author = {Toscano, Miguel and Frecha, Cecilia and Ortega, C. and Santamaría, Manuel and Martín, Francisco and Molina Pineda Infantas, Ignacio Jesús},
}