@misc{10481/101491,
year = {2005},
url = {https://hdl.handle.net/10481/101491},
abstract = {Nonviral producer cell proteins incorporated into retroviral vector surfaces profoundly influence infectivity and in vivo half-life. We report the purification and concentration of lentiviral vectors using these surface proteins as an efficient gene transduction strategy. Biotinylation of these proteins and streptavidin paramagnetic particle concentration enhances titer 400- to 2,500-fold (to 10(9) CFU/ml for vesicular stomatitis virus G protein and 5 x 10(8) for amphotropic murine leukemia virus envelope). This method also uses newly introduced membrane proteins (B7.1 and DeltaLNGFR) directed to lentiviral surfaces, allowing up to 17,000-fold concentrations. Particle conjugation of lentivirus allows facile manipulation in vitro, resulting in the transduction of 48 to 94% of human acute myeloid leukemia blasts.},
publisher = {American Society of Microbiology},
keywords = {gene therapy},
keywords = {targeting},
keywords = {Lentiviral vectors},
keywords = {Myeloid leukemia cells},
keywords = {concentration},
keywords = {Conjugation},
title = {Conjugation of lentivirus to paramagnetic particles via non-viral proteins allows efficient concentration and infection of primary Acute Myeloid Leukaemia cells},
doi = {10.1128/JVI.79.20.13190-13194.2005},
author = {Chan, L and Nesbeth, D and Mackey, t and Galea-Lauri, J and Gaken, J and Mufti, F and Farzaneh, F and Darling, D and Collins, Mary and Martín Molina, Francisco},
}