@misc{10481/101460,
year = {2009},
month = {1},
url = {https://hdl.handle.net/10481/101460},
abstract = {Cultured human embryonic stem (hES) cells can acquire genetic and epigenetic changes that make them vulnerable to transformation. As hES cells with cancer-cell characteristics share properties with normal hES cells, such as self-renewal, teratoma formation and the expression of pluripotency markers, they may be misconstrued as superior hES cells with enhanced 'stemness'. We characterize two variant hES cell lines (v-hESC-1 and v-hESC-2) that express pluripotency markers at high levels and do not harbor chromosomal abnormalities by standard cytogenetic measures. We show that the two lines possess some features of neoplastic progression, including a high proliferative capacity, growth-factor independence, a 9- to 20-fold increase in frequency of tumor-initiating cells, niche independence and aberrant lineage specification, although they are not malignant. Array comparative genomic hybridization reveals an amplification at 20q11.1-11.2 in v-hESC-1 and a deletion at 5q34a-5q34b;5q3 and a mosaic gain of chromosome 12 in v-hESC-2. These results emphasize the need for functional characterization to distinguish partially transformed and normal hES cells.},
title = {Characterization of human embryonic stem cells with features of neoplastic progression},
doi = {10.1038/nbt.1516.},
author = {Werbowetski-Ogilvie, Tamara and Bossé, Marc and Stewart, Morag H and Schnerch, Angelique and Ramos-Mejía, Verónica and Rouleau, Anne and Wynder, Tracy and Smith, Mary-Jo and Dinwall, Steve and Carter, Tim and Williams, Christopher and Harris, Charles and Dolling, Joanna and Wynder, Christopher and Boreham, Doug and Bhatia, Mickie},
}