@misc{10481/101444,
year = {2013},
month = {6},
url = {https://hdl.handle.net/10481/101444},
abstract = {Objectives
There are little data available on the pathology caused by the sibling species Anisakis simplex s.s. and Anisakis pegreffii. The differences shown in their ability to penetrate the muscle of fish may also be manifested in humans. The purpose of this study is to confirm possible differences in pathogenicity between A. simplex s.s. and A. pegreffii using an experimental model which simulates infection in humans.

Methods
Female Wistar rats were infected with 190 Anisakis type I L3 larvae from the Iberian coastline. After the animal was sacrificed, these L3 larvae were then recovered and identified via PCR-RFLP of the ITS1-5.8S-ITS2. A logistic regression analysis was performed searching for association between experimental pathogenic potential and species.

Results
The distribution of A. simplex s.s. and A. pegreffii between Atlantic and Mediterranean waters of the Iberian Peninsula showed statistically significant differences (P < 0.001) which were not observed in the hybrid genotypes (P > 0.3). 21.6% showed pathogenic potential, interpreted as the capacity of the larvae to cause lesions, stick to the gastrointestinal wall or penetrate it. The species variable showed association with the pathogenic role of the larva (P = 0.008). Taking A. simplex s.s. as our reference, the OR for A. pegreffii is 0.351 (P = 0.028).

Conclusions
Despite this difference, A. pegreffii is also capable of causing anisakiasis, being responsible for 14.3% of the penetrations of the gastric mucosa found in rats, which justifies both species being considered aetiologic agents of this parasitic disorder.},
organization = {We are grateful to the Junta de Andalucía (Regional Autonomous Government of Andalusia) for the project grant P07-CVI-03249, and Ictioparasitology group cvi-03249.},
publisher = {WILEY-BLACKWELL PUBLISHING LTD.},
keywords = {Anisakis},
keywords = {Anisakiasis},
keywords = {Anisakis simplex s.s.},
keywords = {Anisakis pegreffii},
keywords = {pathogenic potential},
keywords = {nutrition education},
title = {Experimental comparison of pathogenic potential of twosibling species Anisakis simplex s.s. and Anisakis pegreffii inWistar rat},
doi = {https://doi.org/10.1111/tmi.12131},
author = {Romero López, María del Carmen and Valero López, Adela and Navarro Moll, María Concepción},
}