@misc{10481/101420,
year = {2013},
month = {6},
url = {https://hdl.handle.net/10481/101420},
abstract = {The microbiomes in the gastrointestinal tract (GIT) of individuals receiving antibiotics and those in obese subjects undergo compositional shifts, the metabolic effects and linkages of which are not clearly understood. Herein, we set to gain insight into these effects, particularly with regard to carbohydrate metabolism, and to contribute to unravel the underlying mechanisms and consequences for health conditions. We measured the activity level of GIT carbohydrate-active enzymes toward 23 distinct sugars in adults patients (n = 2) receiving 14-d β-lactam therapy and in obese (n = 7) and lean (n = 5) adolescents. We observed that both 14 d antibiotic-treated and obese subjects showed higher and less balanced sugar anabolic capacities, with 40% carbohydrates being preferentially processed as compared with non-treated and lean patients. Metaproteome-wide metabolic reconstructions confirmed that the impaired utilization of sugars propagated throughout the pentose phosphate metabolism, which had adverse consequences for the metabolic status of the GIT microbiota. The results point to an age-independent positive association between GIT glycosidase activity and the body mass index, fasting blood glucose and insulin resistance (r2 ≥ 0.95). Moreover, antibiotics altered the active fraction of enzymes controlling the thickness, composition and consistency of the mucin glycans. Our data and analyses provide biochemical insights into the effects of antibiotic usage on the dynamics of the GIT microbiota and pin-point presumptive links to obesity. The knowledge and the hypotheses generated herein lay a foundation for subsequent, systematic research that will be paramount for the design of “smart” dietary and therapeutic interventions to modulate host-microbe metabolic co-regulation in intestinal homeostasis.},
organization = {The whole consortium was funded by the Spanish Ministry of Economy and Competitiveness and the Federal Ministry of Education and Research (BMBF) within the ERA NET PathoGenoMics2 program, grant number 0315441A. This work was further funded by grants BFU2008–04501-E, SAF2009–13032-C02–01, SAF2012–31187 and CSD2007–00005 from the Spanish Ministry of Economy and Competitiveness, Prometeo/2009/092 from Generalitat Valenciana (Spain) and AGL2006–11697/ALI. The authors gratefully acknowledge the financial support provided by the European Regional Development Fund (ERDF). This work has been partially supported by EVASYON study funded by the Spanish Ministry of Health and Consumption (Carlos III Institute of Health. FIS Grant PI 051579).},
publisher = {Taylor & Francis},
keywords = {gut microbiota},
keywords = {obesity},
keywords = {antibiotics},
title = {Functional consequences of microbial shifts in the human gastrointestinal tract linked to antibiotic treatment and obesity},
doi = {https://doi.org/10.4161/gmic.25321},
author = {Hernández, Ester and Bargiela, Rafael and Suárez, María and Friedrichs, Anette and Pérez-Cobas, Ana Elena and Gosalbes, María José and Knecht, Henrik and Martínez-Martínez, Mónica and Seifert, Jana and von Bergen, Martin and Artacho, Alejandro and Campoy Folgoso, Cristina and Latorre, Amparo and Ott, Stephan and Moya, Andrés and Suárez García, Antonio Francisco and Martins dos Santos, Vitor and Ferrer, Manuel and Ruiz Rodríguez, Alicia},
}