@misc{10481/101257,
year = {2009},
month = {11},
url = {https://hdl.handle.net/10481/101257},
abstract = {Long Interspersed Element-1 (LINE-1 or L1) retrotransposons have dramatically impacted the
human genome. L1s must retrotranspose in the germ-line or during early development to ensure their
evolutionary success; yet the extent to which this process impacts somatic cells is poorly understood.
We previously demonstrated that engineered human L1s can retrotranspose in adult rat hippocampus
progenitor cells (NPCs) in vitro and in the mouse brain in vivo1. Here, we demonstrate that NPCs
isolated from human fetal brain and NPCs derived from human embryonic stem cells (hESCs) support
the retrotransposition of engineered human L1s in vitro. Furthermore, we developed a quantitative
multiplex polymerase chain reaction that detected an increase in the copy number of endogenous
L1s in the hippocampus and in several regions of adult human brains when compared to the copy
number of endogenous L1s in heart or liver genomic DNAs from the same donor. These data suggest
that de novo L1 retrotransposition events may occur in the human brain and, in principle, have the
potential to contribute to individual somatic mosaicism.},
organization = {F.H.G. and N.G.C. are supported by the Picower
Foundation, Lookout Fund, and the California Institute for Regenerative Medicine (CIRM). J.L.G.P. is supported by
Plan Estabilizacion Grupos SNS ENCYT 2015 (EMER07/56, Instituto de Salud Carlos III, Spain) and through the
IRG-FP7-PEOPLE-2007 Marie Curie program. K.S.O. was supported by grants GM069985 and NS048187 from the
National Institutes of Health. J.V.M. was supported by grants GM082970 and GM069985 from the National Institutes
of Health and by the Howard Hughes Medical Institute.},
title = {L1 retrotransposition in human neural progenitor cells},
doi = {doi: 10.1038/nature08248. Epub 2009 Aug 5.},
author = {Morell Hita, María},
}