Short-term effects of hyaluronic acid on the subgingival microbiome in peri-implantitis: A randomized controlled clinical trial Soriano Lerma, Ana del Carmen Magán Fernández, Antonio Gijón Martín, Juan José Sánchez Fernández, Elena Soriano, Miguel García-Salcedo, José A Mesa Aguado, Francisco Luis Dental Implants Peri-Implantitis Hyaluronic Acid Microbiota Clinical Trial This study was supported by Ricerfarma srl (Milan, Italy) in collaboration with Research Groups #CTS 583 and #BIO-344 (Junta de Andalucía, Spain) (reference: OTRI-3300). Ana Soriano-Lerma was supported by a fellowship from the Ministry of Education, Culture and Sport (FPU 17/05413). José A. García-Salcedo was supported by “Programa Estatal de Inves-tigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad” (grant SAF-SAF2015-71714-RMINECO/FEDER) and The Network of Tropical Diseases Research—RICET (Instituto de Salud Carlos III). Background: The aim of our study was to evaluate the effects of a hyaluronic acid gel at 45 days on the microbiome of implants with peri-implantitis with at least one year of loading. Methods: A randomized controlled trial was conducted in peri-implantitis patients. Swabs containing the samples were collected both at baseline and after 45 days of treatment. 16S rRNA sequencing techniques were used to investigate the effect of hyaluronic acid gel on the subgingival microbiome. Results: 108 samples of 54 patients were analyzed at baseline and after follow-up at 45 days. Three strata with different microbial composition were obtained in the samples at baseline, representing three main microbial consortia associated with peri-implantitis. Stratum 1 did not show any difference for any variable after treatment with hyaluronic acid, whereas in stratum 2, Streptococcus, Veillonella, Rothia and Granulicatella did decrease (p<0.05). Similarly, Prevotella and Campylobacter (p< 0.05) decreased in stratum 3 after treatment with hyaluronic acid. Microbial diversity was found to be decreased in stratum 3 (p<0.05) after treatment with hyaluronic acid compared to the control group, in which an increase was found (p<0.05). Conclusions: Hyaluronic acid reduced the relative abundance of peri-implantitis-related microorganisms, especially the early colonizing bacteria, suggesting a specific action during the first stages in the development of the disease. Hyaluronic acid did not alter relative abundances of non-oral genera. The use of hyaluronic acid in advanced stages of peri-implantitis resulted in a decrease in microbial alpha diversity, suggesting a protective action of the peri-implant site against bacteria colonization. 2024-04-15T09:23:54Z 2024-04-15T09:23:54Z 2020 info:eu-repo/semantics/article Published version: Soriano-Lerma A, Magán-Fernández A, Gijón J, et al. Short-term effectsof hyaluronic acid on the subgingival microbiomein peri-implantitis: A randomized controlled clinicaltrial.J Periodontol. 2020;91:734–745. https://doi.org/10.1002/JPER.19-0184 https://hdl.handle.net/10481/90729 10.1002/JPER.19-0184 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Wiley