Association of muscular strength and targeted proteomics involved in brain health in children with overweight/obesity Olvera-Rojas, Marcos Plaza-Florido, Abel Solís Urra, Patricio Rodríguez Ayllón, María Toval, Angel Esteban-Cornejo, Irene Ortega Porcel, Francisco Bartolomé Muscular strength Biomarkers Brain health Cardiorespiratory fitness Children Proteomics. Muscular strength has been positively associated with better brain health indicators during childhood obesity. However, the molecular mechanisms underlying the positive impact of muscular strength in brain health are poorly understood. We aimed to study the association of muscular strength with neurology-related circulating proteins in plasma in children with overweight/obesity and to explore the role of cardiorespiratory fitness (CRF) as a confounder. The participants were 86 Caucasian children (10.1 ± 1.1 years old; 41% girls) from the ActiveBrains project. Muscular strength was measured by field and laboratory tests. CRF was assessed with an incremental treadmill test. Olink's technology was used to quantify 92 neurology-related proteins in plasma. Protein–protein interactions were computed using the STRING website. Muscular strength was positively associated with 12 proteins (BetaNGF, CDH6, CLEC10A, CLM1, FcRL2, HAGH, IL12, LAIR2, MSR1, SCARB2, SMOC2, and TNFRSF12A), and negatively associated with 12 proteins (CLEC1B, CTSC, CTSS, gal-8, GCP5, NAAA, NrCAM, NTRK2, PLXNB3, RSPO1, sFRP3, and THY1). After adjustment for CRF, muscular strength was positively associated with eight proteins (BetaNGF, CDH6, CLEC10A, FcRL2, LAIR2, MSR1, SCARB2, and TNFRSF12A) and negatively associated with two proteins (gal-8 and NrCAM). After applying FDR correction, only CLEC10A remained statistically significant. In conclusion, muscular strength was associated with blood circulating proteins involved in several biological processes, particularly anti-inflammatory response, lipid metabolism, beta amyloid clearance, and neuronal action potential propagation. More powered studies are warranted in pediatric populations to contrast or confirm our findings. 2023-05-22T10:34:33Z 2023-05-22T10:34:33Z 2023-05-15 info:eu-repo/semantics/article Olvera-Rojas, M., Plaza-Florido, A., Solis-Urra, P., Rodriguez-Ayllon, M., Toval, A., Esteban-Cornejo, I., & Ortega, F. B. (2023). Association of muscular strength and targeted proteomics involved in brain health in children with overweight/obesity. Scandinavian Journal of Medicine & Science in Sports. 37190796 https://hdl.handle.net/10481/81713 https://doi.org/10.1111/sms.14387 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional