Analysis of the Genetic Relationship between Atherosclerosis and Non-Alcoholic Fatty Liver Disease through Biological Interaction Networks Andujar Vera, Francisco Ferrer Millán, Maria García Fontana, Cristina García Fontana, Beatriz González Salvatierra, Sheila Sanabria de la Torre, Raquel Martínez Heredia, Luis Riquelme Gallego, Blanca Muñoz Torres, Manuel Eduardo Atherosclerosis Non-alcoholic fatty liver disease Protein-protein interaction networks Non-alcoholic fatty liver disease (NAFLD) seems to have some molecular links with atherosclerosis (ATH); however, the molecular pathways which connect both pathologies remain un explored to date. The identification of common factors is of great interest to explore some therapeutic strategies to improve the outcomes for those affected patients. Differentially expressed genes (DEGs) for NAFLD and ATH were extracted from the GSE89632 and GSE100927 datasets, and common up- and downregulated DEGs were identified. Subsequently, a protein–protein interaction (PPI) network based on the common DEGs was performed. Functional modules were identified, and the hub genes were extracted. Then, a Gene Ontology (GO) and pathway analysis of common DEGs was performed. DEGs analysis in NAFLD and ATH showed 21 genes that were regulated similarly in both pathologies. The common DEGs with high centrality scores were ADAMTS1 and CEBPA which appeared to be down- and up-regulated in both disorders, respectively. For the analysis of functional modules, two modules were identified. The first one was oriented to post-translational protein modification, where ADAMTS1 and ADAMTS4 were identified, and the second one mainly related to the immune response, where CSF3 was identified. These factors could be key proteins with an important role in the NAFLD/ATH axis. 2023-05-04T08:06:55Z 2023-05-04T08:06:55Z 2023-02-18 info:eu-repo/semantics/article Andújar-Vera, F.; Ferrer-Millán, M.; García-Fontana, C.; García-Fontana, B.; González-Salvatierra, S.; Sanabria-de la Torre, R.; Martínez-Heredia, L.; Riquelme-Gallego, B.; Muñoz-Torres, M. Analysis of the Genetic Relationship between Atherosclerosis and Non-Alcoholic Fatty Liver Disease through Biological Interaction Networks. Int. J. Mol. Sci. 2023, 24, 4124. [https://doi.org/10.3390/ijms24044124] https://hdl.handle.net/10481/81323 10.3390/ijms24044124 eng http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional MDPI