Biomimetic Remineralization of an Extracellular Matrix Collagen Membrane for Bone Regeneration Osorio Ruiz, Raquel Asady, Samara Toledano Osorio, Manuel Toledano Pérez, Manuel Osorio Ruiz, María Estrella Collagen Membrane Bone regeneration Biomineralization Natural extracellular matrix (ECM) collagen membranes are frequently used for bone regeneration procedures. Some disadvantages, such as rapid degradation and questionable mechanical properties, limit their clinical use. These membranes have a heterologous origin and may proceed from different tissues. Biomineralization is a process in which hydroxyapatite deposits mainly in collagen fibrils of the matrices. However, when this deposition occurs on the ECM, its mechanical properties are increased, facilitating bone regeneration. The objective of the present research is to ascertain if different membranes from distinct origins may undergo biomineralization. Nanomechanical properties, scanning electron (SEM) and multiphoton (MP) microscopy imaging were performed in three commercially available ECMs before and after immersion in simulated body fluid solution for 7 and 21 d. The matrices coming from porcine dermis increased their nanomechanical properties and they showed considerable mineralization after 21 d, as observed in structural changes detected through SEM and MP microscopy. It is hypothesized that the more abundant crosslinking and 2022-09-27T08:05:26Z 2022-09-27T08:05:26Z 2022-08-11 info:eu-repo/semantics/article Osorio, R... [et al.]. Biomimetic Remineralization of an Extracellular Matrix Collagen Membrane for Bone Regeneration. Polymers 2022, 14, 3274. [https://doi.org/10.3390/polym14163274] https://hdl.handle.net/10481/77002 10.3390/polym14163274 eng http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional MDPI