Culturing and Molecular Approaches for Identifying Microbiota Taxa Impacting Children’s Obesogenic Phenotypes Related to Xenobiotic Dietary Exposure López Moreno, Ana Ruiz Moreno, Ángel Pardo Cacho, Jesús Cerk, Klara Torres Sánchez, Alfonso Ortiz Sandoval, Pilar Úbeda, Marina Aguilera Gómez, Margarita Culturomics Bioinformatics Obesogens BPA Obesity Endocrine disruptors A.L.-M. has a Ph.D. contract through the EFSA grant and the programme "Intensificacion de la Investigacion" University of Granada (2019-2022). PO is under the contract "Garantia Juvenil" -FEDER-Junta de Andalucia. K. Cerk is under the EU-FORA Fellowship Programme. A.L-M., A.R-M., P.O., A.T.-S. and M.A. are part of the BIO-190 Research Group. They are also part of "UGR Plan Propio de Investigacion 2019-2022. This work was carried out within the frame of FEDER-Infrastructure: IE19_198 UGR and OBEMIRISK EFSA-Partnering Grant Project GP/EFSA/ENCO/2018/03-GA04. Integrated data from molecular and improved culturomics studies might offer holistic insights on gut microbiome dysbiosis triggered by xenobiotics, such as obesity and metabolic disorders. Bisphenol A (BPA), a dietary xenobiotic obesogen, was chosen for a directed culturing approach using microbiota specimens from 46 children with obesity and normal-weight profiles. In parallel, a complementary molecular analysis was carried out to estimate the BPA metabolising capacities. Firstly, catalogues of 237 BPA directed-cultured microorganisms were isolated using five selected media and several BPA treatments and conditions. Taxa from Firmicutes, Proteobacteria, and Actinobacteria were the most abundant in normal-weight and overweight/obese children, with species belonging to the genera Enterococcus, Escherichia, Staphylococcus, Bacillus, and Clostridium. Secondly, the representative isolated taxa from normal-weight vs. overweight/obese were grouped as BPA biodegrader, tolerant, or resistant bacteria, according to the presence of genes encoding BPA enzymes in their whole genome sequences. Remarkably, the presence of sporobiota and concretely Bacillus spp. showed the higher BPA biodegradation potential in overweight/obese group compared to normal-weight, which could drive a relevant role in obesity and metabolic dysbiosis triggered by these xenobiotics. 2022-02-16T08:54:54Z 2022-02-16T08:54:54Z 2022-01-06 info:eu-repo/semantics/article López-Moreno, A... [et al.]. Culturing and Molecular Approaches for Identifying Microbiota Taxa Impacting Children’s Obesogenic Phenotypes Related to Xenobiotic Dietary Exposure. Nutrients 2022, 14, 241. [https://doi.org/10.3390/nu14020241] http://hdl.handle.net/10481/72857 10.3390/nu14020241 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI