PARP inhibition attenuates histopathological lesion in ischemia/reperfusion renal mouse model after cold prolonged ischemia García Del Moral Garrido, Raimundo Gómez-Morales, Mercedes Hernández-Cortés, Pedro Aguilar Peña, David Caballero, Trinidad Aneiros-Fernández, José Caba Molina, Mercedes Rodríguez-Martínez, María Dolores Peralta, Andreina Galindo Moreno, Pablo Antonio Osuna Ortega, Antonio Oliver Pozo, Francisco Javier García Del Moral Garrido, Raimundo O'Valle Ravassa, Francisco Javier Adp-ribose Synthetase Human poly(adp-ribose) Polymerase-1 Reperfusion injury DNA damage Dysfunction Domain Disruption Expression Stress We test the hypothesis that PARP inhibition can decrease acute tubular necrosis (ATN) and other renal lesions related to prolonged cold ischemia/reperfusion (IR) in kidneys preserved at 4°C in University of Wisconsin (UW) solution. Material and Methods. We used 30 male Parp1+/+ wild-type and 15 male Parp10/0 knockout C57BL/6 mice. Fifteen of these wild-type mice were pretreated with 3,4-dihydro-5-[4-(1-piperidinyl)butoxyl]-1(2H)-isoquinolinone (DPQ) at a concentration of 15 mg/kg body weight, used as PARP inhibitor. Subgroups of mice were established (A: IR 45 min/6 h; B: IR + 48 h in UW solution; and C: IR + 48 h in UW solution plus DPQ). We processed samples for morphological, immunohistochemical, ultrastructural, and western-blotting studies. Results. Prolonged cold ischemia time in UW solution increased PARP-1 expression and kidney injury. Preconditioning with PARP inhibitor DPQ plus DPQ supplementation in UW solution decreased PARP-1 nuclear expression in renal tubules and renal damage. Parp10/0 knockout mice were more resistant to IR-induced renal lesion. In conclusion, PARP inhibition attenuates ATN and other IR-related renal lesions in mouse kidneys under prolonged cold storage in UW solution. If confirmed, these data suggest that pharmacological manipulation of PARP activity may have salutary effects in cold-stored organs at transplantation. 2014-05-30T10:42:26Z 2014-05-30T10:42:26Z 2013 info:eu-repo/semantics/article Moral, R.M.G.; et al. PARP inhibition attenuates histopathological lesion in ischemia/reperfusion renal mouse model after cold prolonged ischemia. Scientific World Journal, 2013: 486574 (2013). [http://hdl.handle.net/10481/32049] 1537-744X http://hdl.handle.net/10481/32049 10.1155/2013/486574 eng info:eu-repo/semantics/openAccess Hindawi Publishing Corporation