Culturing and Molecular Approaches for Identifying Microbiota Taxa Impacting Children’s Obesogenic Phenotypes Related to Xenobiotic Dietary Exposure
Metadatos
Mostrar el registro completo del ítemAutor
López Moreno, Ana; Ruiz Moreno, Ángel; Pardo Cacho, Jesús; Cerk, Klara; Torres Sánchez, Alfonso; Ortiz Sandoval, Pilar; Úbeda, Marina; Aguilera Gómez, MargaritaEditorial
MDPI
Materia
Culturomics Bioinformatics Obesogens BPA Obesity Endocrine disruptors
Fecha
2022-01-06Referencia bibliográfica
López-Moreno, A... [et al.]. Culturing and Molecular Approaches for Identifying Microbiota Taxa Impacting Children’s Obesogenic Phenotypes Related to Xenobiotic Dietary Exposure. Nutrients 2022, 14, 241. [https://doi.org/10.3390/nu14020241]
Patrocinador
OBEMIRISK EFSA-Partnering Grant GP/EFSA/ENCO/2018/03-GA04; FEDER-Infrastructure IE19_198 UGRResumen
Integrated data from molecular and improved culturomics studies might offer holistic insights
on gut microbiome dysbiosis triggered by xenobiotics, such as obesity and metabolic disorders.
Bisphenol A (BPA), a dietary xenobiotic obesogen, was chosen for a directed culturing approach
using microbiota specimens from 46 children with obesity and normal-weight profiles. In parallel,
a complementary molecular analysis was carried out to estimate the BPA metabolising capacities.
Firstly, catalogues of 237 BPA directed-cultured microorganisms were isolated using five selected
media and several BPA treatments and conditions. Taxa from Firmicutes, Proteobacteria, and Actinobacteria
were the most abundant in normal-weight and overweight/obese children, with species
belonging to the genera Enterococcus, Escherichia, Staphylococcus, Bacillus, and Clostridium. Secondly,
the representative isolated taxa from normal-weight vs. overweight/obese were grouped as BPA
biodegrader, tolerant, or resistant bacteria, according to the presence of genes encoding BPA enzymes
in their whole genome sequences. Remarkably, the presence of sporobiota and concretely Bacillus
spp. showed the higher BPA biodegradation potential in overweight/obese group compared to
normal-weight, which could drive a relevant role in obesity and metabolic dysbiosis triggered by
these xenobiotics.