@misc{10481/90205,
year = {2017},
month = {2},
url = {https://hdl.handle.net/10481/90205},
abstract = {Aims: To determine the usefulness of the fluorescence parameters generated by Sysmex UF-1000i flow cytometer for the rapid diagnosis of urinary tract infection by bacilli or cocci.

Methods and results: Urine samples (n = 1924) were studied by culture and microbiology and subsequently by cytometry, using BACT-Morph software and considering forward-scattered light (FSC) and fluorescent light scatter fluorescence parameters. BACT-Morph software showed moderate diagnostic accuracy (78·4%) to detect rod-shaped bacteria, with sensitivity of 82·4% and specificity of 62·5%. Forward-scattered (B_FSC) values of the bacterial channel were significant higher for the Gram-positive cocci category (P < 0·001). A cut-off of B_FSC ≥24·2, expressed in arbitrary units (analytical channel, ch), provided higher sensitivity (90·0%) but lower specificity (38·9%), and the diagnostic accuracy for Gram-positive cocci classification reached 62·0%.

Conclusions: Utilization of BACT-Morph software and bacterial channel fluorescence parameters (B_FSC ≥24·2 ch) offered an approximate discrimination of bacilli and cocci but the specificity was low, especially for FSC.

Significance and impact of the study: Further research is needed to establish the usefulness of flow cytometry for aetiological diagnosis.},
publisher = {Oxford University Press},
keywords = {Diagnosis},
keywords = {Fluorescence},
keywords = {Urinary tract infection},
title = {Comparison between urine culture profile and morphology classification using fluorescence parameters of the Sysmex UF-1000i urine flow cytometer},
doi = {10.1111/jam.13354},
author = {Jiménez-Guerra, Gemma and Heras-Cañas, Víctor and Valera-Arcas, M.D. and Rodríguez-Granger, Javier and Navarro, J.M. and Gutiérrez Fernández, José},
}