@misc{10481/89519,
year = {2024},
month = {1},
url = {https://hdl.handle.net/10481/89519},
abstract = {Salivary pH serves as a valuable and useful diagnostic marker for periodontal disease, as it not only plays a critical role in disease prevention but also in its development. Typically, saliva sampling is collected by draining and spitting it into collec- tion tubes or using swabs. In this study, we have developed a Point-of-Care (POC) device for in situ determination of oral pH without the need for complex instruments, relying solely on a smartphone as the detection device. Our system utilizes a non-toxic vegetable colourimetric indicator, immobilized on a chitosan membrane located on a disposable stick, enabling direct sampling within the buccal cavity. An ad hoc designed 3D-printed attachment is used to ensure accurate positioning and alignment of the stick, as well as isolation from external lighting conditions. A custom-developed smartphone application captures and automatically processes the image of the sensing membrane, providing the salivary pH results. After optimiz- ing the cocktail composition, the developed sensors demonstrated the capacity to determine pH within a range of 5.4 to 8.1 with a remarkable precision of 0.6%, achieving a very short analysis time of just 1 min. A stability study conducted on the sensing membranes revealed a lifetime of 50 days. To validate the performance of our analytical device, we compared its results against those obtained from a calibrated pH-meter, using a group of individuals. The device exhibited an average error of 2.4% when compared with the pH-meter results, confirming its reliability and accuracy},
organization = {Funding for open access publishing: Universidad de Gra- nada/CBUA. This work was funded by Spanish MCIN (Project PID2019-103938RB-I00, Project PID2022-138727OB-I00 funded by MCIN/AEI/10.13039/501100011033 and, by ERDF “A way of making Europe”, by the “European Union” and grant IJC2020- 043307-I) and Junta de Andalucía (Projects B-FQM-243-UGR18, P18-RT-2961). The projects and the grant were partially supported by European Regional Development Funds (ERDF) and by European Union NextGenerationEU/PRTR.},
keywords = {Colourimetric sensors},
keywords = {Smartphone color detection},
keywords = {Salivary pH determination},
keywords = {Anthocyanins},
keywords = {Poin-of-Care (POC)},
title = {POC device for rapid oral pH determination based on a smartphone platform},
doi = {https://doi.org/10.1007/s00604-024-06227-1},
author = {Arroyo, Manuel J. and Escobedo Araque, Pablo and Ruiz García, Isidoro and Palma López, Alberto José and Santoyo González, Francisco and Ortega Muñoz, Mariano and Capitán Vallvey, Luis Fermín and Erenas Rodríguez, Miguel María},
}