@misc{10481/89496, year = {2013}, month = {6}, url = {https://hdl.handle.net/10481/89496}, abstract = {We are developing a cultivation method with the aim to achieve the complete lifecycle of Anisakis in the laboratory using only culture media. We study the development, ecdysis and survival of A. simplex to know the factors that are involved in the growth and survival of this parasitic nematode. The use of calf’s liver infusion in the media for cultivation of Anisakis simplex causes a positive effect since M4 molting occurs earlier, and it also increases the survival and the size of the worms.}, publisher = {Facultad de Ciencias de la Salud. Universidad de Granada}, keywords = {Anisakiasis}, keywords = {Cultivation}, keywords = {Culture media}, keywords = {Life cycle}, keywords = {Anisakis simplex}, title = {In vitro cultivation of Anisakis simplex, causal agent of the human anisakiasis}, author = {Soria Soto, Manuel and Molina Fernández, Dolores and Hidalgo Hidalgo, Verónica and Adroher Auroux, Francisco Javier and Benítez Rodríguez, Rocío and Malagón Martínez, David}, }