@misc{10481/88521,
year = {2011},
month = {2},
url = {https://hdl.handle.net/10481/88521},
abstract = {Protein from porcine blood meal was hydrolysed with Alcalase to obtain a final revalorised product suitable, for example, to take part in the composition of an organic fertiliser. Three experimental factors of the reaction (pH, temperature and enzyme-substrate ratio) were optimised by means of a statistically designed experiment and response surface methodology. The goal of the optimisation problem was to maximise both the degree of hydrolysis and solubilisation of the substrate, obtaining a maximum degree of hydrolysis (28.89%) with pH 6.24, 54.2 °C and enzyme-substrate ratio of 10%. Regarding the content of suspended solids, its minimum value (30.29% related to the initial weight of blood meal) was attained at pH 7.5, 59.8 °C and enzyme-substrate ratio of 10%. The controversial effects of pH and temperature on the substrate solubilisation and the final degree of hydrolysis, suggested employing a multiobjective optimisation technique. A Pareto Front was generated in order to find a set of intermediate solutions which satisfied both objectives in an adequate degree.},
organization = {Ministerio de Ciencia e Innovación ( CTQ2008-02978)},
publisher = {ELSEVIER},
keywords = {Blood meal},
keywords = {Enzyme bioreactors},
keywords = {Modelling},
keywords = {Bi-objective optimisation},
keywords = {Proteolysis},
keywords = {Response surface methodology},
title = {BI-OBJECTIVE OPTIMISATION OF THE ENZYMATIC HYDROLYSIS OF PORCINE BLOOD PROTEIN},
doi = {https://doi.org/10.1016/j.bej.2010.12.004},
author = {Pérez Gálvez, Antonio Raúl and Almecija Rodríguez, María Carmen and Espejo Carpio, Francisco Javier and Guadix Escobar, Antonio María and Guadix Escobar, Emilia María},
}