@misc{10481/86343,
year = {2021},
month = {1},
url = {https://hdl.handle.net/10481/86343},
abstract = {Non-alcoholic fatty liver disease (NAFLD) has reached pandemic proportions worldwide.
We have previously reported that the probiotic strains Bifidobacterium breve CNCM I-4035,
Lactobacillus paracasei CNCM I-4034 and Lactobacillus rhamnosus CNCM I-4036 exert anti-inflammatory
effects in the intestine of Zucker-Leprfa/fa rats. In this work, we focused on their hepatic effects.
M1 macrophages are related to inflammation and NAFLD pathogenesis, whereas M2 macrophages
release anti-inflammatory mediators. We evaluated the effects of these 3 strains on macrophage
polarization, inflammation and liver damage of Zucker-Leprfa/fa rats. The animals received either a
placebo or 1010 CFU of probiotics orally for 30 days. Nos2 and Cd86 mRNA levels were determined
as markers of M1 macrophages, and Cd163 and Arg1 as M2 markers, respectively, by qRT-PCR. Liver
damage was determined by lipid peroxidation, leukocyte infiltration and myeloperoxidase activity.
We evaluated a panoply of circulating chemokines, the hepatic ratio P-Akt/Akt, NF-kB and P-NF-kB
protein levels. All 3 probiotic strains modulated macrophage polarization in liver and circulating
levels of inflammation-related mediators. L. paracasei CNCM I-4034 increased the ratio P-Akt/Akt
and NF-kB protein levels. B. breve CNCM I-4035, L. paracasei CNCM I-4034 and L. rhamnosus CNCM
I-4036 decreased both pro-inflammatory macrophage gene expression and leukocyte infiltration in
the liver.},
organization = {Hero Spain, S. A. (grant #3545 managed by
Fundación General Empresa-Universidad de Granada)},
organization = {Grant #PI-0538–2017 from Junta de Andalucía, Spain},
publisher = {MDPI},
keywords = {Macrophage},
keywords = {Non-alcoholic fatty liver disease},
title = {Bifidobacterium breve CNCM I-4035, Lactobacillus paracasei CNCM I-4034 and Lactobacillus rhamnosus CNCM I-4036 Modulate Macrophage Gene Expression and Ameliorate Damage Markers in the Liver of Zucker-Leprfa/fa Rats.},
doi = {10.3390/nu13010202},
author = {Fontana Gallego, Luis and Plaza Díaz, Julio and Robles Bolívar, Paula and Sáez Lara, María José and Abadía Molina, Francisco and Gómez Llorente, Carolina and Gil Hernández, Ángel and Álvarez Mercado, Ana Isabel},
}