@misc{10481/56613,
year = {2018},
month = {6},
url = {http://hdl.handle.net/10481/56613},
abstract = {This study is a comparison of the efficiency of three technologies used for Y chromosome capture and
the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our
main findings disclose that streptavidin–biotin magnetic particle-based capture methodology offers
better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting
and microdissection procedures. Moreover, this methodology is less time consuming and the most
selective for capturing only Y chromosomal material, in contrast with other methodologies that result
in considerable background material from other, non-targeted chromosomes. NGS results compared
between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the
first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our
results indicate an improved strategy for Y chromosome research with applications in several scientific
fields where this chromosome plays an important role, such as forensics, medical sciences, molecular
anthropology and cancer sciences.},
organization = {Spanish Alfonso Martin Escudero Foundation for the financial support to one of the
authors of the present work (MJ Alvarez –Cubero).},
publisher = {Springer Nature},
title = {Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads},
doi = {10.1038/s41598-018-27819-x},
author = {Álvarez Cubero, María Jesús and Santiago, O. and Martínez Labarga, Cristina and Martínez-García, B. and Marrero-Díaz, R. and Rubio-Roldán, A. and Pérez-Gutiérrez, A. M. and Carmona Sáez, Pedro and Lorente Acosta, José Antonio and Martínez González, Luis Javier},
}