TY  - JOUR 
AU  - Gutiérrez Rus, Luis Ignacio
AU  - Risso, Valeria Alejandra
AU  - Sánchez Ruiz, José Manuel
PY  - 2022
UR  - https://hdl.handle.net/10481/76988
AB  - The routine generation of enzymes with completely new active sites is a major unsolved problem in protein engineering. Advances in this field have thus far been modest, perhaps due, at least in part, to the widespread use of modern natural proteins as...
LA  - eng
PB  - MDPI
KW  - Enzyme design
KW  - De novo enzymes
KW  - Kemp elimination
KW  - Ancestral enzymes
KW  - Beta-lactamases
KW  - Focused library screening
KW  - Focused directed evolution
TI  - Efficient Base-Catalyzed Kemp Elimination in an Engineered Ancestral Enzyme
DO  - 10.3390/ijms23168934
ER  -