TY  - JOUR 
AU  - Perazzoli, Gloria
AU  - Prados Salazar, José Carlos
AU  - Ortiz Quesada, Raúl
AU  - Caba Pérez, Octavio
AU  - Cabeza Montilla, Laura
AU  - Berdasco, María
AU  - González Astorga, Beatriz
AU  - Melguizo Alonso, Consolación
PY  - 2015
SN  - 1932-6203
UR  - http://hdl.handle.net/10481/38652
AB  - [Background]

The use of temozolomide (TMZ) has improved the prognosis for glioblastoma multiforme patients. However, TMZ resistance may be one of the main reasons why treatment fails. Although this resistance has frequently been linked to the...
AB  - [Methods]

Four nervous system tumor cell lines were used to analyze the modulation of MGMT expression and MGMT promoter methylation by TMZ treatment. Furthermore, 5-aza-2’-deoxycytidine was used to demethylate the MGMT promoter and...
AB  - [Results]

Our results showed two clearly differentiated groups of tumor cells characterized by low (A172 and LN229) and high (SF268 and SK-N-SH) basal MGMT expression. Interestingly, cell lines with no MGMT expression and low TMZ IC50 showed a high...
AB  - [Conclusions]

These results may be relevant in understanding the phenomenon of TMZ resistance, especially in glioblastoma multiforme patients laking MGMT expression, and may also aid in the design of new therapeutic strategies to improve the...
LA  - eng
PB  - Public Library of Science (PLOS)
KW  - Cancer treatment
KW  - DNA methylation
KW  - Gene expression
KW  - Cancer stem cells
KW  - Cell cycle and cell division
KW  - G1 phase
KW  - G2 phase
KW  - Glioblastoma multiforme
TI  - Temozolomide Resistance in Glioblastoma Cell Lines: Implication of MGMT, MMR, P-Glycoprotein and CD133 Expression
DO  - 10.1371/journal.pone.0140131
ER  -