TY  - JOUR 
AU  - Carreras Egaña, Francisco Javier
AU  - Aranda, Carlos J.
AU  - Porcel, David
AU  - Rodríguez-Hurtado, Francisco
AU  - Martínez-Agustín, Olga
AU  - Zarzuelo Zurita, Antonio
PY  - 2015
UR  - http://hdl.handle.net/10481/37092
AB  - Background:

To develop the use of cultured tissue of the prelaminar optic nerve of the pig to explore possible alterations of the astrocyte-axon metabolic pathways in glaucoma, we map the distribution of the glucose transporters GLUT1 and GLUT3 in...
AB  - Methods:

We monitor cell survival in cultures of the prelaminar optic-nerve tissue, measuring necrosis and apoptosis markers biochemically as well as morphologically, and establish the presence of the glucose transporters GLUT1 and GLUT3. We map...
AB  - Results:

We find that the main death type in prelaminar culture is apoptosis. Caspase 7 staining reveals an increment in apoptosis from day 1 to day 4 and a reduction from day 4 to day 8. Western blotting for GLUT1 shows stability with increased...
AB  - Conclusions:

Tissue culture is suitable for apoptosis-induction experiments. The results suggest that glucose is transported to the axonal cleft intracytoplasmically and delivered to the cleft by GLUT1 transporters. As monocarboxylate transporters...
LA  - eng
PB  - Public Library of Science (Plos)
KW  - Astrocytes
KW  - Apoptosis
KW  - Glucose
KW  - Axons
KW  - Confocal laser microscopy
KW  - Transmission electron microscopy
KW  - DAPI staining
KW  - Glucose metabolism
TI  - Expression of Glucose Transporters in the Prelaminar Region of the Optic-Nerve Head of the Pig as Determined by Immunolabeling and Tissue Culture
DO  - 10.1371/journal.pone.0128516
ER  -