In vitro cultivation of Anisakis simplex, causal agent of the human anisakiasis Soria Soto, Manuel Molina Fernández, Dolores Hidalgo Hidalgo, Verónica Adroher Auroux, Francisco Javier Benítez Rodríguez, Rocío Malagón Martínez, David Anisakiasis Cultivation Culture media Life cycle Anisakis simplex We are developing a cultivation method with the aim to achieve the complete lifecycle of Anisakis in the laboratory using only culture media. We study the development, ecdysis and survival of A. simplex to know the factors that are involved in the growth and survival of this parasitic nematode. The use of calf’s liver infusion in the media for cultivation of Anisakis simplex causes a positive effect since M4 molting occurs earlier, and it also increases the survival and the size of the worms. 2024-02-23T09:43:27Z 2024-02-23T09:43:27Z 2013-06 info:eu-repo/semantics/bookPart Soria-Soto, M., Molina-Fernández, D., Hidalgo-Hidalgo, V., Adroher, F. J., Benítez, R., & Malagón, D. (2013). In vitro cultivation of Anisakis simplex, causal agent of the human anisakiasis. In: Facultad de Ciencias de la Salud, & Universidad de Granada (Eds.), Nuevos retos en investigación, docencia y clínica en Ciencias de la Salud (pp. 223–227). 978-84-15450-15-3 https://hdl.handle.net/10481/89496 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Facultad de Ciencias de la Salud. Universidad de Granada