Biotechnological and protein-engineering implications of ancestral protein resurrection Risso, Valeria Alejandra Sánchez Ruiz, José Manuel Ozkan, S. Banu Ancestral environments likely differed from the environments hosting modern proteins. Resurrected ancestral protein often display unusual properties and interactions. Approximations to the sequences of ancestral proteins can be derived from the sequences of their modern descendants. Proteins encoded by such reconstructed sequences can be prepared in the laboratory and subjected to experimental scrutiny. These ‘resurrected’ ancestral proteins often display remarkable properties, reflecting ancestral adaptations to intra-cellular and extra-cellular environments that differed from the environments hosting modern/extant proteins. Recent experimental and computational work has specifically discussed high stability, substrate and catalytic promiscuity, conformational flexibility/diversity and altered patterns of interaction with other sub-cellular components. In this review, we discuss these remarkable properties as well as recent attempts to explore their biotechnological and proteinengineering potential. 2024-01-22T13:11:28Z 2024-01-22T13:11:28Z 2018-08 info:eu-repo/semantics/article Published version: http://dx.doi.org/10.1016/j.sbi.2018.02.007 https://hdl.handle.net/10481/87099 10.1016/j.sbi.2018.02.007 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional