Consolidation of degraded ornamental porous limestone stone by calcium carbonate precipitation induced by the microbiota inhabiting the stone Jiménez López, Concepción Rodríguez Navarro, Carlos Manuel Piñar, Guadalupe Carrillo Rosúa, Francisco Javier Rodríguez Gallego, Manuel González Muñoz, María Teresa Bacterial biomineralization Biomineralizaciones bacterianas Stone conservation Conservación de la piedra Myxococcus xanthus Calcium carbonate Carbonato cálcico El artículo original ha sido publicado por Chemosphere, de la Editorial Elsevier, disponible en: http://www.elsevier.com/wps/find/journaldescription.cws_home/362/description#description http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6V74-4NF2NCX-1&_user=654849&_coverDate=08%2F31%2F2007&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000035398&_version=1&_urlVersion=0&_userid=654849&md5=a44718f997b5057999cd6a5f100b7289&searchtype=a Although it has already been shown that calcareous stone can be consolidated by using a bacterially-inoculated culture media, a more user-friendly method is the in situ application of a sterile culture media that is able to activate, among the microbial community of the stone, those bacteria with a potential for calcium carbonate precipitation. In order to test this new method for stone consolidation, non-sterilized decayed porous limestone was immersed in sterile nutritional media. Results were compared to those of the runs in which stone sterilized prior to the treatment was used. The effects of the microbial community on stone consolidation were determined by recording the evolution of the culture media chemistry. The treated stones were tested for mechanical resistance and porosity. Results demonstrate that the tested media were able to activate bacteria from the microbial community of the stone. As a consequence of the growth of these bacteria, an alkalinization occurred that resulted in calcium carbonate precipitation. The new precipitate was compatible with the substrate and consolidated the stone without pore plugging. Therefore, a good candidate to in situ consolidate decayed porous limestone is the application of a sterile culture media with the characteristics specified in the present study. 2011-01-25T10:58:28Z 2011-01-25T10:58:28Z 2007 info:eu-repo/semantics/article Jimenez-Lopez, C., Rodriguez-Navarro, C., Piñar, G., Carrillo-Rosúa, J., Rodriguez-Gallego, M., & Gonzalez-Muñoz, M. T. (2007). Consolidation of degraded ornamental porous limestone stone by calcium carbonate precipitation induced by the microbiota inhabiting the stone. Chemosphere, 68(10), 1929-1936. doi:10.1016/j.chemosphere.2007.02.044 0045-6535 http://hdl.handle.net/10481/7428 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Elsevier