Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells Fueyo González, Francisco Espinar Barranco, Laura Crovetto González, Luis Paredes Martínez, José Manuel Orte Gutiérrez, Ángel González Vera, Juan Antonio Glutathione Luminescent sensor Self-assembled antenna Lanthanide Time-resolved luminescence Flow cytometry T cells TREG This work was supported by grants CTQ2017-85658-R, BFU2015-67284-R, and PID2019-104366RB-C22 funded by MCIN/AEI/10.13039/501100011033/FEDER "Una manera de hacer Europa"; grant PID2020-114256RB-I00 funded by MCIN/AEI/10.13039/501100011033; grant A-FQM-386-UGR20 funded by FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades, and the CSIC grant 201580E073. Funding for open access charge: Universidad de Granada/CBUA. The small molecule 8-methoxy-2-oxo-1,2,4,5- tetrahydrocyclopenta[de]quinoline-3-carboxylic acid (2b) behaves as a reactive non-fluorescent Michael acceptor, which after reaction with thiols becomes fluorescent, and an efficient Eu3+ antenna, after self-assembling with this cation in water. This behavior makes 2b a highly selective GSH biosensor, which has demonstrated high potential for studies in murine and human cells of the immune system (CD4+ T, CD8+ T, and B cells) using flow cytometry. GSH can be monitored by the fluorescence of the product of addition to 2b (445 nm) or by the luminescence of Eu3+ (592 nm). 2b was able to capture baseline differences in GSH intracellular levels among murine and human CD4+ T, CD8+ T, and B cells. We also successfully used 2b to monitor intracellular changes in GSH associated with the metabolic variations governing the induction of CD4+ naiv̈ e T cells into regulatory T cells (TREG). 2022-02-04T11:35:42Z 2022-02-04T11:35:42Z 2022-01-15 info:eu-repo/semantics/article ACS Sens. 2022, 7, 1, 322–330. [https://doi.org/10.1021/acssensors.1c02439] http://hdl.handle.net/10481/72669 10.1021/acssensors.1c02439 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España American Chemical Society