Selecting FRET pairs for visualizing amyloid aggregation Ruiz Arias, Álvaro Jurado Palomares, Rocío Fueyo Gonzalez, Francisco Herranz, Rosario Gálvez Rodríguez, Natividad González Vera, Juan Antonio Orte Gutiérrez, Ángel Amyloid fibrils Protein aggregation Solvatochromic dyes Biomarkers FLIM STED microscopy This work was supported by grant CTQ2017–85658-R funded by MCIN/AEI/10.13039/501100011033/FEDER “Una manera de hacer Europa” and grants PID2019–104366RB-C22 and PID2020–114256RBI00 funded by MCIN/AEI/10.13039/ 501100011033. A.R.-A. thanks the Spanish Ministerio de Educación y Formación Profesional for an FPU Ph.D. studentship. In a recent work, we reported a methodology for imaging the different stages of amyloid aggregation in quantitative multiparametric dual-color fluorescence lifetime imaging (FLIM) and superresolution microscopy by using a pair of dyes capable of binding aggregates and undergoing subsequent intra-aggregate energy transfer (FRET) (RuizArias et al. Sensors Actuat. B, 2022, 350:130882). In this microarticle we present the optimization process for choosing the best pair of dyes through a screening of different naphthalimides and quinolimides and other known amyloid-binding dyes. 2022-02-01T11:59:50Z 2022-02-01T11:59:50Z 2021-12-30 info:eu-repo/semantics/article A. Ruiz-Arias et al. Selecting FRET pairs for visualizing amyloid aggregation. Results in Chemistry 4 (2022) 100275 [https://doi.org/10.1016/j.rechem.2021.100275] http://hdl.handle.net/10481/72590 10.1016/j.rechem.2021.100275 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Elsevier