Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads Álvarez Cubero, María Jesús Santiago, O. Martínez Labarga, Cristina Martínez-García, B. Marrero-Díaz, R. Rubio-Roldán, A. Pérez-Gutiérrez, A. M. Carmona Sáez, Pedro Lorente Acosta, José Antonio Martínez González, Luis Javier This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin–biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences. 2019-08-09T07:40:23Z 2019-08-09T07:40:23Z 2018-06-21 info:eu-repo/semantics/article Álvarez-Cubero, María Jesús; et. al. Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads. Scientific Reports (2018) 8:9436 [DOI:10.1038/s41598-018-27819-x] http://hdl.handle.net/10481/56613 10.1038/s41598-018-27819-x eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Springer Nature