Physiological responses to acute cold exposure in young lean men Acosta Manzano, Francisco Miguel Martínez Téllez, Borja Manuel Sánchez-Delgado, Guillermo Alcantara, Juan M.A. Acosta Manzano, Pedro Morales-Artacho, Antonio J. Ruiz Ruiz, Jonatan The aim of this study was to comprehensively describe the physiological responses to an acute bout of mild cold in young lean men (n = 11, age: 23 ± 2 years, body mass index: 23.1 ± 1.2 kg/m2) to better understand the underlying mechanisms of non-shivering thermogenesis and how it is regulated. Resting energy expenditure, substrate metabolism, skin temperature, thermal comfort perception, superficial muscle activity, hemodynamics of the forearm and abdominal regions, and heart rate variability were measured under warm conditions (22.7 ± 0.2ÊC) and during an individualized cooling protocol (air-conditioning and water cooling vest) in a cold room (19.4 ± 0.1ÊC). The temperature of the cooling vest started at 16.6ÊC and decreased ~ 1.4ÊC every 10 minutes until participants shivered (93.5 ± 26.3 min). All measurements were analysed across 4 periods: warm period, at 31% and at 64% of individualÂs cold exposure time until shivering occurred, and at the shivering threshold. Energy expenditure increased from warm period to 31% of cold exposure by 16.7% (P = 0.078) and to the shivering threshold by 31.7% (P = 0.023). Fat oxidation increased by 72.6% from warm period to 31% of cold exposure (P = 0.004), whereas no changes occurred in carbohydrates oxidation. As shivering came closer, the skin temperature and thermal comfort perception decreased (all P<0.05), except in the supraclavicular skin temperature, which did not change (P>0.05). Furthermore, the superficial muscle activation increased at the shivering threshold. It is noteworthy that the largest physiological changes occurred during the first 30 minutes of cold exposure, when the participants felt less discomfort. 2019-07-12T09:02:27Z 2019-07-12T09:02:27Z 2018 info:eu-repo/semantics/article Acosta FM, Martinez-Tellez B, Sanchez- Delgado G, A. Alcantara JM, Acosta-Manzano P, Morales-Artacho AJ, et al. (2018) Physiological responses to acute cold exposure in young lean men. PLoS ONE 13(5): e0196543. https://doi.org/ 10.1371/journal.pone.0196543 1932-6203 http://hdl.handle.net/10481/56399 eng http://creativecommons.org/licenses/by-nc/3.0/es/ info:eu-repo/semantics/openAccess Atribución-NoComercial 3.0 España Public Library of Science