Mechanistic Differences in Neuropathic Pain Modalities Revealed by Correlating Behavior with Global Expression Profiling Cobos del Moral, Enrique José Nickerson, Chelsea A. Gao, Fuying Chandran, Vijayendran Bravo Caparrós, Inmaculada González-Cano, Rafael Riva, Priscilla Andrews, Nick A. Latremoiere, Alban Seehus, Corey R. Perazzoli, Gloria Nieto López, Francisco Rafael Joller, Nicole Painter, Michio W. Ma, Chi Him Eddie Omura, Takao Chesler, Elissa J. Geschwind, Daniel H. Coppola, Giovanni Rangachari, Manu Woolf, Clifford J. Costigan, Michael Neuropathic pain Pain macrophages T cells Immune system TrpV1 Tactile allodynia Cold allodynia Gene expression Transcript profiling WCGNA Chronic neuropathic pain is a major morbidity of neural injury, yet its mechanisms are incompletely understood. Hypersensitivity to previously non-noxious stimuli (allodynia) is a common symptom. Here, we demonstrate that the onset of cold hypersensitivity precedes tactile allodynia in a model of partial nerve injury, and this temporal divergence was associated with major differences in global gene expression in innervating dorsal root ganglia. Transcripts whose expression change correlates with the onset of cold allodynia were nociceptor related, whereas those correlating with tactile hypersensitivity were immune cell centric. Ablation of TrpV1 lineage nociceptors resulted in mice that did not acquire cold allodynia but developed normal tactile hypersensitivity, whereas depletion of macrophages or T cells reduced neuropathic tactile allodynia but not cold hypersensitivity. We conclude that neuropathic pain incorporates reactive processes of sensory neurons and immune cells, each leading to distinct forms of hypersensitivity, potentially allowing drug development targeted to each pain type. 2018-02-16T13:04:19Z 2018-02-16T13:04:19Z 2018-01 info:eu-repo/semantics/article Cobos, E.J.; et al. Mechanistic Differences in Neuropathic Pain Modalities Revealed by Correlating Behavior with Global Expression Profiling. Cell Reports, 22(5): 1301-1312 (2018). [http://hdl.handle.net/10481/49592] 2211-1247 http://hdl.handle.net/10481/49592 10.1016/j.celrep.2018.01.006 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Cell Press