Zinc-modified nanopolymers improve the quality of resin-dentin bonded interfaces Osorio Ruiz, Raquel Cabello Malagón, Inmaculada Medina Castillo, Antonio Luis Osorio Ruiz, María Estrella Toledano Pérez, Manuel Remineralization Zinc Nanopolymers Dentin Hybrid layer Adhesives Introduction: Demineralized collagen fibers at the hybrid layer are susceptible to degradation. Remineralization may aid to improve bond longevity. Objectives: The aim of the present study was to infiltrate zinc and calcium-loaded polymeric nanoparticles into demineralized dentin to facilitate hybrid layer remineralization. Materials and methods: Zinc or calcium-loaded polymeric nanoparticles were infiltrated into etched dentin, and Single Bond Adhesive was applied. Bond strength was tested after 24 h and 6 months storage. Nanomechanical properties, dyeassisted confocal laser microscopy, and Masson’s trichrome staining evaluation were performed to assess for the hybrid layer morphology, permeability, and remineralization ability after 24 h and 3 months. Data were analyzed by ANOVA and Student–Newman–Keuls multiple comparisons tests (p < 0.05). Results: Immediate bond strength was not affected by nanoparticles infiltration (25 to 30 MPa), while after 6 months, bond strengths were maintained (22 to 24 MPa). After 3 months, permeability occurred only in specimens in which nanoparticles were not infiltrated. Dentin remineralization, at the bottom of the hybrid layer, was observed in all groups. After microscopy analysis, zinc-loaded nanoparticles were shown to facilitate calcium deposition throughout the entire hybrid layer. Young’s modulus at the hybrid layer increased from 2.09 to 3.25 GPa after 3 months, in specimens with zinc nanoparticles; meanwhile, these values were reduced from 1.66 to 0.49 GPa, in the control group. Conclusion: Infiltration of polymeric nanoparticles into demineralized dentin increased long-term bond strengths. Zinc-loaded nanoparticles facilitate dentin remineralization within the complete resin–dentin interface. Clinical relevance: Resin–dentin bond longevity and dentin remineralization at the hybrid layer were facilitated by zincloaded nanoparticles. 2017-06-07T08:33:25Z 2017-06-07T08:33:25Z 2016-01-30 info:eu-repo/semantics/article Osorio Ruiz, R.; et al. Zinc-modified nanopolymers improve the quality of resin-dentin bonded interfaces. Clinical Oral Investigations, 20(9): 2411-2420 (2016). [http://hdl.handle.net/10481/46693] 1432-6981 1436-3771 http://hdl.handle.net/10481/46693 10.1007/s00784-016-1738-y eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Springer International Publishing