Silk fibroin nanoparticles constitute a vector for controlled release of resveratrol in an experimental model of inflammatory bowel disease in rats Lozano-Pérez, Antonio Abel Rodríguez-Nogales, Alba Ortiz-Cullera, Víctor Algieri, Francesca Garrido-Mesa, José Zorrilla, Pedro Rodríguez Cabezas, María Elena Garrido-Mesa, Natividad Utrilla Navarro, Pilar Matteis, Laura de Martínez de la Fuente, Jesús Cenis, José Luis Gálvez Peralta, Julio Juan Immunomodulatory Cytokines TNBS rat colitis Antioxidants RAW 264.7 macrophage cells Purpose: We aimed to evaluate the intestinal anti-inflammatory properties of silk fibroin nanoparticles, around 100 nm in size, when loaded with the stilbene compound resveratrol, in an experimental model of rat colitis. Methods: Nanoparticles were loaded with resveratrol by adsorption. The biological effects of the resveratrol-loaded nanoparticles were tested both in vitro, in a cell culture of RAW 264.7 cells (mouse macrophages), and in vivo, in the trinitrobenzenesulfonic acid model of rat colitis, when administered intracolonically. Results: The resveratrol liberation in 1× phosphate-buffered saline (PBS; pH 7.4) was characterized by fast liberation, reaching the solubility limit in 3 hours, which was maintained over a period of 80 hours. The in vitro assays revealed immunomodulatory properties exerted by these resveratrol-loaded nanoparticles since they promoted macrophage activity in basal conditions and inhibited this activity when stimulated with lipopolysaccharide. The in vivo experiments showed that after evaluation of the macroscopic symptoms, inflammatory markers, and intestinal barrier function, the fibroin nanoparticles loaded with resveratrol had a better effect than the single treatments, being similar to that produced by the glucocorticoid dexamethasone. Conclusion: Silk fibroin nanoparticles constitute an attractive strategy for the controlled release of resveratrol, showing immunomodulatory properties and intestinal anti-inflammatory effects. 2014-10-20T10:27:32Z 2014-10-20T10:27:32Z 2014 info:eu-repo/semantics/article Lozano-Pérez, A.A.; et al. Silk fibroin nanoparticles constitute a vector for controlled release of resveratrol in an experimental model of inflammatory bowel disease in rats. International Journal of Nanomedicine, 9(1): 4507-4520 (2014). [http://hdl.handle.net/10481/33442] 1178-2013 http://hdl.handle.net/10481/33442 10.2147/IJN.S68526 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Dove Press