Antigen Incorporation on Cryptosporidium parvum Oocyst Walls Entrala Torres, Emilio Sbihi, Younes Sánchez Moreno, Manuel Mascaró Lazcano, María Carmen Cryptosporidium Oocyst wall Antigens Cryptosporidium parvum oocysts are the infective stages responsible for transmission and survival of the organism in the environment. In the present work we show that the oocyst wall, far from being a static structure, is able to incorporate antigens by a mechanism involving vesicle fusion with the wall, and the incorporation of the antigen to the outer oocyst wall. Using immunoelectron microscopy we show that the antigen recognized by a monoclonal antibody used for diagnosis of cryptosporidiosis (MerifluorÒ, Meridian Diagnostic Inc.) could be found associated with vesicles in the space between the sporozoites and the oocysts wall, and incorporated to the outer oocyst wall by an unknown mechanism. 2014-07-04T10:21:23Z 2014-07-04T10:21:23Z 2001 info:eu-repo/semantics/article Entrala, E.; et al. Antigen Incorporation on Cryptosporidium parvum Oocyst Walls. Memórias do Instituo Oswaldo Cruz, 96(2): 233-235 (2001). [http://hdl.handle.net/10481/32451] 0074-0276 http://hdl.handle.net/10481/32451 10.1590/S0074-02762001000200016 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Instituto Oswaldo Cruz