Estrogenicity of resin-based composites and sealants used in dentistry Olea Serrano, Nicolás Pulgar Encinas, Rosa María Pérez, Pilar Olea Serrano, Fátima Rivas, A. Novillo-Fertrell, Arancha Pedraza Muriel, Vicente Soto, Ana M. Sonnenschein, Carlos Bisphenol-A Composite resins and sealants E-screen bioassay Restorative dentistry Xenoestrogens We tested some resin-based composites used in dentistry for their estrogenic activity. A sealant based on bisphenol-A diglycidylether methacrylate (bis-GMA) increased cell yields, progesterone receptor expression, and pS2 secretion in human estrogen-target, serum-sensitive MCF7 breast cancer cells. Estrogenicity was due to bisphenol-A and bisphenol-A dimethacrylate, monomers found in the base paste of the dental sealant and identified by mass spectrometry. Samples of saliva from 18 subjects treated with 50 mg of a bis-GMA-based sealant applied on their molars were collected 1 hr before and after treatment. Bisphenol-A (range 90-931 micrograms) was identified only in saliva collected during a 1-hr period after treatment. The use of bis-GMA-based resins in dentistry, and particularly the use of sealants in children, appears to contribute to human exposure to xenoestrogens. 2014-07-02T10:37:57Z 2014-07-02T10:37:57Z 1996 info:eu-repo/semantics/article Olea, N.; et al. Estrogenicity of resin-based composites and sealants used in dentistry. Environmental Health Perspectives, 104(3): 298-305 (1996). [http://hdl.handle.net/10481/32434] 0091-6765 http://hdl.handle.net/10481/32434 10.2307/3432888 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License National Institute of Environmental Health