The Regulatory Subunit of PKA-I Remains Partially Structured and Undergoes β-Aggregation upon Thermal Denaturation Dao, Khanh K. Pey Rodríguez, Ángel Luis Gjerde, Anja Underhaug Teigen, Knut Byeon, In-Ja L. Døskeland, Stein O. Gronenborn, Angela M. Martínez, Aurora Atomic force microscopy Biochemical simulations Cricular dichroism spectroscopy Crystal structure Fluorescence Fluorescence microscopy Protein structure Thermal stability [Background] The regulatory subunit (R) of cAMP-dependent protein kinase (PKA) is a modular flexible protein that responds with large conformational changes to the binding of the effector cAMP. Considering its highly dynamic nature, the protein is rather stable. We studied the thermal denaturation of full-length RIα and a truncated RIα(92-381) that contains the tandem cyclic nucleotide binding (CNB) domains A and B. [Methodology/Principal Findings] As revealed by circular dichroism (CD) and differential scanning calorimetry, both RIα proteins contain significant residual structure in the heat-denatured state. As evidenced by CD, the predominantly α-helical spectrum at 25°C with double negative peaks at 209 and 222 nm changes to a spectrum with a single negative peak at 212–216 nm, characteristic of β-structure. A similar α→β transition occurs at higher temperature in the presence of cAMP. Thioflavin T fluorescence and atomic force microscopy studies support the notion that the structural transition is associated with cross-β-intermolecular aggregation and formation of non-fibrillar oligomers. [Conclusions/Significance] Thermal denaturation of RIα leads to partial loss of native packing with exposure of aggregation-prone motifs, such as the B' helices in the phosphate-binding cassettes of both CNB domains. The topology of the β-sandwiches in these domains favors inter-molecular β-aggregation, which is suppressed in the ligand-bound states of RIα under physiological conditions. Moreover, our results reveal that the CNB domains persist as structural cores through heat-denaturation. 2014-03-26T12:37:13Z 2014-03-26T12:37:13Z 2011 info:eu-repo/semantics/article Dao, K.K.; et al. The Regulatory Subunit of PKA-I Remains Partially Structured and Undergoes β-Aggregation upon Thermal Denaturation. Plos One, 6(3): e17602 (2011). [http://hdl.handle.net/10481/31128] 1932-6203 doi: 10.1371/journal.pone.0017602 http://hdl.handle.net/10481/31128 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Public Library of Science (PLOS)