Early Stage Biomineralization in the Periostracum of the ‘Living Fossil’ Bivalve Neotrigonia Checa González, Antonio G. Salas, Carmen Harper, Elizabeth M. Bueno-Pérez, Juan de Dios Basal cells Bivalves Calcification Crystals Microvilli Prisms Secretion Vesicles A detailed investigation of the shell formation of the palaeoheterodont ‘living fossil’ Neotrigonia concentrated on the timing and manufacture of the calcified ‘bosses’ which stud the outside of all trigonioid bivalves (extant and fossil) has been conducted. Electron microscopy and optical microscopy revealed that Neotrigonia spp. have a spiral-shaped periostracal groove. The periostracum itself is secreted by the basal cell, as a thin dark pellicle, becoming progressively transformed into a thin dark layer by additions of secretions from the internal outer mantle fold. Later, intense secretion of the internal surface of the outer mantle fold forms a translucent layer, which becomes transformed by tanning into a dark layer. The initiation of calcified bosses occurred at a very early stage of periostracum formation, deep within the periostracal groove immediately below the initialmost dark layer. At this stage, they consist of a series of polycyclically twinned crystals. The bosses grow as the periostracum traverse through the periostracal groove, in coordination with the thickening of the dark periostracal layer and until, upon reaching the mantle edge, they impinge upon each other and become transformed into large prisms separated by dark periostracal walls. In conclusion, the initial bosses and the external part of the prismatic layer are fully intraperiostracal. With later growth, the prisms transform into fibrous aggregates, although the details of the process are unknown. This reinforces the relationships with other groups that have the ability to form intraperiostracal calcifications, for example the unionoids with which the trigonioids form the clade Paleoheterodonta. The presence of similar structures in anomalodesmatans and other euheterodonts raises the question of whether this indicates a relationship or represents a convergence. The identification of very early calcification within an organic sheet has interesting implications for our understanding of how shells may have evolved. 2014-03-25T10:22:13Z 2014-03-25T10:22:13Z 2014 info:eu-repo/semantics/article Checa, A.; Salas, C.; Harper, E.M.; Bueno-Pérez, J.D. Early Stage Biomineralization in the Periostracum of the ‘Living Fossil’ Bivalve Neotrigonia. Plos One, 9(2): e90033 (2014). [http://hdl.handle.net/10481/31084] 1932-6203 doi: 10.1371/journal.pone.0090033 http://hdl.handle.net/10481/31084 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Public Library of Science (PLOS)