Mineralization of human premolar occlusal fissures: a quantitative histochemical microanalysis Campos Muñoz, Antonio Jesús Rodríguez, Ismael Ángel Sánchez Quevedo, María Del Carmen García López, José Manuel Nieto Albano, O. H. Gómez de Ferraris, María Elsa Mineralization Premolar Fissures Microanalysis The mechanisms of cariogenesis in occlusal fissures remain elusive because of limited information about fissure structure and wall mineralization. The purpose of the present study was to determine the correlation between morphological patterns in occlusal fissures in human premolars and quantitative histochemical patterns of mineralization in the walls of these formations. We used scanning electron microscopy and quantitative X-ray microanalysis with the peak-tolocal background ratio method and microcrystalline calcium salts as standards. We distinguished three morphological patterns of fissures in scanning electron microscopic images. The wall of the fissures was less mineralized than the control enamel in all three types of fissures. Because the fissure walls are hypomineralized, we suggest that practicing dentists should take into account the degree of mineralization when they are preparing the fissures for the application of sealant. 2014-03-10T11:53:51Z 2014-03-10T11:53:51Z 2000 info:eu-repo/semantics/article Campos, A.; et al. Mineralization of human premolar occlusal fissures: a quantitative histochemical microanalysis. Histology and Histopathology, 15(2): 499-502 (2000). [http://hdl.handle.net/10481/30760] 0213-3911 http://hdl.handle.net/10481/30760 eng http://hdl.handle.net/10201/19309 http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Universidad de Murcia